نتایج جستجو برای: multiplex ligation dependentprobe amplification

تعداد نتایج: 97340  

Journal: :The Analyst 2013
Jeongkyeong Na Gi Won Shin Gyu Yong Jung Gyoo Yeol Jung

Aberrant DNA methylation is a potential diagnostic marker for complex diseases, such as cancer. With the increase in the number of genes known to exhibit disease-associated aberrant methylation, the need for accurate multiplex assays for quantifying DNA methylation has increased. Methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) is one method that has been highligh...

2008
Jordy Coffa Mark A. van de Wiel Begoña Diosdado Beatriz Carvalho Jan Schouten Gerrit A. Meijer

BACKGROUND Multiplex Ligation dependent Probe Amplification (MLPA) is a rapid, simple, reliable and customized method for detection of copy number changes of individual genes at a high resolution and allows for high throughput analysis. This technique is typically applied for studying specific genes in large sample series. The large amount of data, dissimilarities in PCR efficiency among the di...

Journal: :Nucleic Acids Research 2005
Anders O. H. Nygren Najim Ameziane Helena M. B. Duarte Raymon N. C. P. Vijzelaar Quinten Waisfisz Corine J. Hess Jan P. Schouten Abdellatif Errami

Copy number changes and CpG methylation of various genes are hallmarks of tumor development but are not yet widely used in diagnostic settings. The recently developed multiplex ligation-dependent probe amplification (MLPA) method has increased the possibilities for multiplex detection of gene copy number aberrations in a routine laboratory. Here we describe a novel robust method: the methylatio...

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