BACKGROUND In this study, we evaluated the analytical performance and clinical potential of a one-step multiplex real-time PCR assay for the simultaneous detection of 14 types of respiratory viruses using the AdvanSure RV real-time PCR Kit (LG Life Sciences, Korea). METHODS Three hundred and twenty clinical specimens were tested with the AdvanSure RV real-time PCR Kit and conventional multipl...

This paper reports on the experimental evaluation of a Graspable User Interface that employs a "spacemultiplexing" input scheme in which each function to be controlled has a dedicated physical transducer, each occupying its own space. This input style contrasts the more traditional "time-multiplexing" input scheme which uses one device (such as the mouse) to control different functions at diffe...

A new method based on least square support vector machine (LSSVM) combined with FastICA is proposed to extract the fetal electrocardiogram (FECG) from the abdominal signals of a pregnant woman. Firstly, the LSSVM is applied to estimate the maternal electrocardiogram (MECG) component in the multiplex abdominal signals. Then the optimal estimation of multiplex noise-added FECG is obtained by remo...

SARAVANAN, M., M. PARTHIBAN, P. RAMADASS: Genotyping of rotavirus of neonatal calves by nested-multiplex PCR in India. Vet. arhiv 76, 497-505, 2006. ABSTRACT A total of 209 diarrhoeic faecal samples were collected from calves in nine diary cattle herds located in different areas in Tamil Nadu state, India. Nine faecal samples positive for group A rotavirus by polyacrylamide gel electrophoresis ...

A multiplex PCR was developed to detect the coagulase gene (coa; pathognomic of Staphylococcus aureus) and the mecA gene (characteristically encoding for methicillin resistance in staphylococci) in a single, rapid test. Suitable primers for the gene targets and an internal, amplification control were incorporated into a multiplex PCR assay, which was then optimized on a capillary air thermal cy...

We present an exact mathematical framework able to describe site-percolation transitions in real multiplex networks. Specifically, we consider the average percolation diagram valid over an infinite number of random configurations where nodes are present in the system with given probability. The approach relies on the locally treelike ansatz, so that it is expected to accurately reproduce the tr...

Simple and reliable conventional multiplex-polymerase chain reaction (multiplex-PCR) as well as real-time PCR approaches for the partial-length cytochrome b or cyt b gene of mitochondrial DNA (mtDNA) were compared to identify seven animal species for the authenticity of low non Halal (pig, dog, cat and donkey) and high value Halal (cattle, sheep and goat) meats. Either analysis indicated the su...

This paper describes a single tube assay for 20 fluorescent labelled Y-STR loci, which is rapid and robust state-of-art multiplex system. Reaction conditions, including annealing temperature, concentration of primers, magnesium and template DNA, DNA polymerase and reaction volume, were optimised to yield robust amplification. The assay could withstand moderate fluctuations in reaction parameter...

Multiple pilosebaceous cysts include the entities of steatocystoma multiplex and eruptive vellus hair cysts (EVHCs). Multiple pilosebaceous cysts are proposed to be one entity originating in the pilosebaceous duct, since steatocystoma multiplex and EVHCs are frequently present concomitantly and are caused by a cystic change in the same pilosebaceous duct. Here, we describe a patient with yellow...

Multiplex Manager 1.0 is a user-friendly cross-platform program that designs efficient combinations of existing genetic marker loci into multiplex polymerase chain reactions and optimizes using prior marker information. The program has the flexibility to solve two design problems: combining all markers into the smallest number of reactions, or alternatively, selecting a subset from many availab...