Precise quantification of molecular targets in a biological sample across wide dynamic range is key requirement many diagnostic procedures, such as monitoring response to therapy or detection measurable residual disease. State the art digital PCR assays provide for four orders magnitude. However are complex and require sophisticated microfluidic tools. Here we present an assay format that enabl...

interest in hydroxy - carboxylic acid 2 (hca2) receptor has been raised since it is the target of antidyslipidemic drug nicotinic acid. the present study aimed to evaluate the presence of mrna of this receptor in different organs of laboratory rat. twenty two different organs of rats including mesenteric fat, epididymis (head, body and tail), testis, ovary, xiphoid process, liver, adrenal gland...

runx3, a member of runt-related transcription factor (runx) proteins with tumor suppressor effect, is a tissue–restricted and cancer related transcription factor that regulate cell proliferation and growth, as well as differentiation. in the present study, exogenous run3 was transiently expressed in ags (human gastric adenocarcinoma), with undetectable runx3 protein and in a549 (human lung carc...

to make an accurate estimation of the uptake of radioactivity in an organ using the conjugate view method, corrections of physical factors, such as background activity, scatter, and attenuation are needed. the aim of this study was to evaluate the accuracy of four different methods for background correction in activity quantification of the heart in myocardial perfusion scans. the organ activit...

PURPOSE The predictive role of the quantification of thymidylate synthase (TS) in tumors treated with antifolate drugs, such as 5-fluorouracil (5-FU), has been extensively reported in a variety of human tumors. Neuroendocrine tumors (NET) represent potential targets of antifolate agents, but no data on TS expression level in these tumors are currently available. EXPERIMENTAL DESIGN A series o...

RATIONALE AND OBJECTIVE Vascular cell adhesion molecule-1 (VCAM-1) is upregulated in ischemia reperfusion injury (IRI), persisting after restoration of blood flow. We hypothesized that microparticles of iron oxide targeting VCAM-1 (VCAM-MPIO) would depict "ischemic memory" and enable in vivo assessment of VCAM-1 expression. METHODOLOGY AND FINDINGS Mice subject to unilateral, transient (30 mi...