BACKGROUND The aim of this study was to evaluate the non-genomic action of thyroxin on sperm kinetic and its probable use to improve sperm recovery after applying an en- richment method like "swim-up" in comparison with the available one, pentoxifylline. MATERIALS AND METHODS This is an experimental study. A total of 50 patients were re- cruited, followed by infertility consultation. Conventi...

Probiotics have been proposed as alternatives to pharmacological products in several medical conditions including the modulation of obesity, which is frequently associated with poor semen quality. However, effects of probiotics on male fertility have been less investigated. This study assessed the effect of Lactobacillus rhamnosus PB01 (DSM-14870) on sperm kinematic parameters in Normal-weight ...

The aim of this study was to identify different motile sperm subpopulations in ejaculates from an autochthonous bull breed (Bos taurus) and to determine possible modifications in these subpopulations resulting from cryopreservation. Ejaculates were collected and cryopreserved following a conventional protocol. The overall sperm motility and the kinematic parameters of individual spermatozoa wer...

The aims of the present study were: (1) to determine the existence of sperm subpopulations with specific motility characteristics in fresh ejaculates from Holstein bulls, (2) to investigate the effects of semen cryopreservation and post-thaw incubation on the distribution of spermatozoa within the different subpopulations, and (3) to evaluate the existence of between-bull variation in the sperm...

Fertilization is a multistep process requiring spermatozoa with unique cellular structures and numerous germ cell-specific molecules that function in the various steps. In the highly coordinated process of male germ cell development, RNA splicing and polyadenylation help regulate gene expression to assure formation of functional spermatozoa. Male germ cells express tauCstF-64 (Cstf2t gene produ...

The relationship between the three basic parameters of ejaculated spermatozoa, i.e. concentration, motility and morphology, and the results of intracytoplasmic sperm injection (ICSI) were investigated in 838 microinjection cycles. A further 123 ICSI treatment cycles in which testicular spermatozoa were used for microinjection were also evaluated. The influence of anti-sperm antibodies (ASA) on ...

In this study, quantitive characteristics and chemical composition of in roach (Rutilus rutilus caspicus) sperm were investigated. Sperm traits included sperm movement duration, percentage of motile spermatozoa, sperm density, spermatocrit and sperm volume. Some seminal plasma characteristics (sodium, potassium, calcium, magnesium and chloride) were investigated. In addition, some metabolites o...

Fertilization is a cell-cell recognition process that occurs between two distinct cells: a small asymmetric and motile sperm cell and a large and nonmotile egg. The stages of fertilization can be divided into four processes: 1) sperm preparation, 2) sperm-egg recognition and binding, 3) sperm-egg fusion and 4) fusion of sperm and egg pronuclei and activation of the zygote. The specific structur...

Changes in sperm features during the movement phase are especially interesting to study in external fertilization species whose sperm duration movement is long because this implies a significant adaptation of moving cells to the external medium. This study describes the changes in tetraploid Pacific oyster sperm characteristics in relation to time post activation. Sperm individually collected o...

Background: There is a general deterioration in sperm quality depending on time postmortem. Refrigeration of the epididymes down to around 5°C is the best strategy to lower this damage and there are many dissim ilarities between species, possibly due to differences on cold shock endurance of epididymal sperm. Our objective was to determine the effect of 5°C and 37°C on goat epididymal sperms mo...