Mosquito inoculation is a highly sensitive technique for isolation and titration of dengue virus (DENV) from sera, human tissues, wild animals, or mosquitoes. It has been under utilized since it was described 40 years ago because most dengue laboratories do not have access to an insectary to rear mosquitoes. This technique requires good eye-hand coordination while doing manipulation under a ste...

The selection of an appropriate oviposition site is crucial to the maternal fitness of eggraft producing mosquitoes. Females in this group of mosquitoes produce a single egg-raft during their lifetimes. All egg-raft producing mosquitoes are members of the subfamily Culicinae and include the medically important genera Culex and Culiseta. Egg-raft producing mosquitoes use several physical and che...