The multipotent hemopoietic stem cell has fastidious growth requirements in vitro. Traditionally, phytohemagglutinin-stimulated leukocyte conditioned medium has been used to supply the undefined growth factors required for culture of the human multipotent hemopoietic progenitor. We describe the use of medium conditioned by the bladder carcinoma cell line, 5637, to replace PHA-LCM in CFU-GEMM cu...

The effect of conditioned medium on the growth rate of bovine aorta endothelial cells (CSC311) was examined. Media conditioned by several cell types (both immortal and normal) was found to increase the growth rate of CSC311 cells. The growth rate of CSC311 was increased even when the medium was conditioned by other CSC311 cells. The rate of growth of unstimulated CSC311 cells is 0.43 population...

large number of clinical cases in endodontics, however, cytotoxicity of such sealers must be tested before their validation as fi lling materials. the aim of this study was to evaluate the cytotoxic effect of a new copaiba oil-based root canal sealer (biosealer [bs]) on osteoblast-like osteo-1 cells. materials and methods: the experimental groups were formed according to the culture medium cond...

Spinal motoneurons from chick embryos were purified by retrograde transport and fluorescence-activated cell sorting. Growth conditions for motoneurons were studied, with experiments focused on the effects of conditioned media from chick myotubes, fibroblasts, and spinal cord dividing cells. Motoneurons rapidly extended neurites when plated onto polylysine-coated dishes that had been exposed to ...

Monitoring of inflammation in adipose tissues, which causes insulin resistance, is valuable in evaluating insulin resistance. We developed an in vitro analysis system using a fluorescence protein (FP) as a reporter gene driven by pro-inflammatory cytokine promoters such as monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor-α (TNFα). In the reporter-transfected RAW264 cells, th...

Activin-A has a mitogenic effect on vascular smooth muscle cells (SMCs) and is produced by monocyte/macrophage lineage cells. Here, we studied the expression of follistatin, an activin-binding protein, in both A10 cells, a rat aortic SMC line, and vascular SMCs derived from adult rat aorta. Follistatin mRNA was detected in these cells by Northern blot analysis. Ligand blot and immunoblot analys...

The human amniotic membrane known as the innermost single epithelial-covered layer provides many applications such as applicable anti-inflammatory and anti-cancer effects. These immunomodulatory effects belongs to the epithelial cells, a type of epiblast-derived fetal stem cells which currently used for regenerative medicine and transplantation. These cells are collected by author-prepared faci...

The human promyelocytic leukemic cell line HL-60 can be induced to mature monocytes and macrophages in vitro by lymphocyte-conditioned medium. We are reporting sequential changes in surface antigenic expressions, which are sensitive markers of the characteristic events in the process of cell differentiation. The promyelocyte membrane antigen, detected by a monoclonal antibody produced using HL-...