The local viscoelasticity was compared between full-length and telopeptide-removed collagen solutions using optical-tweezers-based microrheology. An enhanced modulus at higher concentrations in full-length collagen solutions suggests contributions from telopeptide-associated transient crosslinks. OCIS codes: (160.1435) Biomaterials; (160.5470) Polymers; (350.4855) Optical tweezers or optical ma...

This paper presents an improved direct binary search (DBS)-based algorithm for generating holograms to holographic optical tweezers. The simulations show that the improved algorithm greatly enhances computation speed while maintaining high hologram efficiency and high-intensity homogeneous target spots. The improved algorithm was applied to generate holographic optical tweezers in several exper...

This study applies a novel computational method to study molecular recognition for three sets of synthetic hosts: molecular clips, molecular tweezers, and a synthetic barbiturate receptor. The computed standard free energies of binding for the 12 binding reactions agree closely with experiment and provide insight into the roles of configurational entropy, preorganization, and induced fit in the...

This chapter overviews the current state of the emerging discipline of 4 DNA nanorobotics that make use of synthetic DNA to self-assemble operational 5 molecular-scale devices. Recently there have been a series of quite astonishing 6 experimental results—which have taken the technology from a state of intriguing 7 possibilities into demonstrated capabilities of quickly increasing scale and com8...

This chapter overviews the current state of the emerging discipline of DNA nanorobotics that make use of synthetic DNA to self-assemble operational molecular-scale devices. Recently there have been a series of quite astonishing experimental results which have taken the technology from a state of intriguing possibilities into demonstrated capabilities of quickly increasing scale and complexity. ...

Cells employ a variety of strategies to maintain proteome homeostasis. Beginning during protein biogenesis, the translation machinery and a number of molecular chaperones promote correct de novo folding of nascent proteins even before synthesis is complete. Another set of molecular chaperones helps to maintain proteins in their functional, native state. Polypeptides that are no longer needed or...

Upon light irradiation, photochromic materials exhibit drastic changes in absorbance addition to molecular and mechanical changes. In this study, we demonstrated optical trapping of microcrystals a double fiber tweezers system combined with two external lights for photoisomerization observed the force by photoisomerization. situ photoisomerizations single crystal trapped were performed thrice r...

An optical tweezers apparatus uses a tightly focused laser to generate a trapping force that can capture and move small particles under a microscope. Because it can precisely and non-destructively manipulate objects such as individual cells and their internal components, the optical tweezers is extremely useful in biological physics research. In this experiment the student will use optical twee...