Fluorescence microscopic approaches represent powerful techniques to monitor molecular interactions in the cellular milieu. Measurements of fluorescence lifetime and anisotropy enjoy considerable popularity in this context. These measurements are often performed on live as well as fixed cells. We report here that formaldehyde-induced cell fixation introduces heterogeneities in the fluorescence ...

A method for measuring photo-fluorescence decay times to approximately Observations are made of the phase and modulation of the light from a 7.5 Mc/s air discharge tube, and of the fluorescence excited by this light, using a photomultiplier modulated at 15 Mc/s. The decay times of anthracene, stilbene, terphenyl and diphenylacetylene crystals of various thicknesses have been measured. Due t o t...

Cutting-edge biophysical technologies including total internal reflection fluorescence microscopy, single molecule fluorescence, single channel opening events, fluorescence resonance energy transfer, high-speed exposures, two-photon imaging, fluorescence lifetime imaging, and other tools are becoming increasingly important in immunology as they link molecular events to cellular physiology, a ke...

Abstract The SiO 2 layer fabricated by the atomic deposition (ALD) method was applied to control efficiency of fluorescence quenching graphene oxide (GO) in supported lipid bilayers (SLBs). SLB formed vesicle fusion on ALD-SiO GO deposited thermally oxidized /Si substrates. Wide-field observation layers with various thicknesses showed that varied reasonable distance dependence expected from equ...

Today, fluorescence imaging spans wide spectrum in modern biological investigations from molecular to network level at submicron resolution. To take advantage of full potential of advanced fluorescence imaging techniques, it is important to understand some underlying physical principles. This chapter provides a framework to understand basic principles underlying fluorescence, fluorescence micro...