Numerous instances of reverse transcriptase (RT) inhibition of the PCR were observed while developing nonquantitative uncoupled RT-PCR techniques for detecting nitrogenase and ammonia monooxygenase gene expression in situ. The inhibitory effect of RT on the PCR was removed with increasing template concentrations beyond 10(5) to 10(6) copies. Including T4 gene 32 protein during the reverse trans...

Few studies have analysed the depth distribution of marine zooplankton at high-resolution, and knowledge obtained from theoretical modelling studies predominates over that from empirical field studies. We analysed depth selection by the marine copepod Calanus finmarchicus during spring and summer in neritic and oceanic Subarctic regions. Data on hydrography and zooplankton distribution in the u...

The human population has been growing at least at the rate of 1.2% annually since 1960 reachingup to 6.4B in 2002 and more than 7.0B in 2012. In Davao City, the annual population growth rate is running at 3.22%in year 1990, at 2.41% last 2007 and recently in 2013 at 2.36%. The alarming problem we confront today is thatthe increase in land area for human use does not expand in proportion to popu...

The potential impacts of land use on large woody debris (LWD) were examined in Sourdough Creek Watershed, a rapidly growing area encompassing Bozeman, Montana, USA. We identified six land classes within a 250 m buffer extending on either side of Sourdough Creek and assessed aquatic habitat and geomorphologic variables within each class. All LWD pieces were counted, and we examined 14 other vari...

tajikistan is a hotspot of plant and animal species diversity and endemism andis important for the conservation of biodiversity on a global scale. the country is located ata biological crossroads. species from central and northern europe, central asia, themiddle east, and north africa mingle here with endemics found nowhere else. therichness of tajikistan‘s biodiversity shows up at the genetic,...

this study was performed in order to evaluate the effects of application of super absorbent on the yield and water use efficiency of cucumber under deficit irrigation conditions in greenhouse. plants cultivated in plastic pots and irrigation emitter with four liters per hour discharge was performed. three levels of irrigation water height (10, 8.3 and 6.2 mm day-1) were used as the main factor ...