Nuclear magnetic resonance (NMR) studies of large membrane-associated proteins are limited by the difficulties in preparation of stable protein-detergent mixed micelles and by line broadening, which is typical of these macroassemblies. We have used the 68-kDa homotetrameric KcsA, a thermostable N-terminal deletion mutant of a bacterial potassium channel from Streptomyces lividans, as a model sy...

Reversed micellar two-phase extraction is a developing technique for protein separation. Introduction of an affinity ligand is considered to be an effective approach to increase the selectivity and capacity of reversed micelles. In this article, Cibacron Blue F3G-A (CB) as an affinity ligand was immobilized to reversed micelles composed of soybean lecithin by a two-phase reaction. The affinity ...

Higher steryl ester biosynthetic activities were obtained with Triton X-100-phosphatidylcholine-cholesterol mixed micelles than with Tween 80-phosphatidylcholine-cholesterol mixed micelles when incubated with spinach leaf (Spinacia oleracea L.) acetone powder preparations. The best incorporation of [4-(14)C]cholesterol into [4-(14)C]cholesteryl ester was obtained with a Triton X-100-phosphatidy...

The interactions of cationic starch (CS, degree of substitution=0.8)/mixed anionic surfactant/water systems were investigated. The surfactants investigated were sodium dodecyl sulphate (SDS), sodium octanoate (NaOct), sodium decanoate (NaDe), sodium dodecanoate (NaDod), sodium oleate (NaOl ) and sodium erucate (NaEr). The concentration of CS varied from 0.001 to 10 wt.%. The effect of mixing on...

The mechanism of vesicle-to-micelle or micelle-to-vesicle transition was studied in order to control sizes and fluidities of vesicles during periods of preparation. Dependence of particle sizes measured by quasi-elastic light scattering, turbidities, fluidity parameters monitored by ESR spectroscopy, and morphological changes of mixed aggregates of egg yolk phosphatidylcholine (EPC) and a deter...

We report the formation and characterisation of easily functionalisable mixed micelles with DNA/PEO corona and PPO core which can be loaded with hydrophobic molecules and stabilised by the formation of a cross-linked semi-interpenetrating network. Furthermore, the corona is functionalised by hybridisation either with dye-modified complementary DNA, with demonstrable distance control, or with DN...

Using complementary physical-chemical methods including turbidimetry, quasielastic light scattering, gel filtration, and phase analysis, we examined the interactions between dilute concentrations of the common bile salt, taurochenodeoxycholate (TCDC), and uni- and multilamellar vesicles (MLVs) composed of defined molecular species of lecithin (L) and varying contents of cholesterol (Ch). Dissol...