During the course of identifications mosquitoes collected around Finland in summer 2022 an adult Culex male from southwestern coast was immediate interest due to its noticeable morphological differences recorded fauna. Upon examination, genitalia keyed modestus, and subsequent sequences barcoding region Cytochrome c oxidase subunit I (COI) confirmed identification. This is first finding Cx. mod...

Cytochrome c oxidase from bakers’ yeast contains three mitochondrially made subunits (I to III) which are relatively hydrophobic and four cytoplasmically made subunits (IV to VII) which are relatively hydrophilic (Mason. T. L., Poyton, R. O., Wharton, D. C., and Schatz, G. (1973) J. Biol. Chem. 248, 1346-1354 and Poyton, R. O., and Schatz, G. (1975) J. Biol. Chem. 250, 752-761. In order to expl...

Background: Traditionally, morphological features of Rhipicephalus (Boophilus) annulatus from closely-related ticks have been considered for their identification and differentiation. However, it is difficult and requires expertise in order to accurately identify and differentiate engorged female ticks and some developmental stages such as larva and nymph from other similar ticks. Hence, molecul...

Three distinct types of terminal oxidases participate in the aerobic respiratory pathways of Paracoccus denitrificans. Two alternative genes encoding sub unit I of the aa3 -type cytochrome c oxidase have been isolated before, namely ctaDI and ctaDII. Each of these genes can be expressed separately to complement a double mutant (ActaDI, ActaDII), indicating that they are isoforms of subunit I of...

theplant pathogenic phytophthora drechsleri is morphologically similar to some other non-papillate phytophthora spp., especially p. melonis, and it is difficult to discriminate these convergent taxa. it seems that the iranian putative p. drechsleri isolates from different cucurbit species have been generally misidentified and their characteristics do not match with p. drechsleri. in order to co...

Various human diseases are associated with mitochondrial DNA (mtDNA) mutations, but heteroplasmy—the coexistence of mutant and wild-type mtDNA—complicates their study. We previously isolated a temperature-lethal mtDNA mutation in Drosophila, mt:CoI(T300I), which affects the cytochrome c oxidase subunit I (CoI) locus. In the present study, we found that the decrease in cytochrome c oxidase (COX)...

Two patients with direct tandem duplications of mitochondrial DNA (mtDNA) and mitochondrial myopathy are described. The breakpoint regions between duplicated segments were amplified using the polymerase chain reaction (PCR), cloned and sequenced. The distribution of normal and abnormal genomes in different tissues was investigated using Southern hybridisation, and in different cells within the ...

Ellobium chinense is a red-listed snail species of the family Ellobiidae with geographic distribution from Vietnam and south coast China to South Korea mainland Japan. This restricted specialized habitats in narrow upper-intertidal lower-supratidal zone salt marshes thus particularly sensitive environmental degradation through land reclamation other human activities. Here, we first report genet...

In the Xingu river basin, Brazil, occurs two genera of Potamotrygonidae family: Potamotrygon and Paratrygon. this region, taxa have significant economic importance for ornamental fishing industry, being intensively captured, especially species leopoldi, which is endemic to basin. attempt propose a species-specific DNA marker from Xingu, as well ensuring robust reliable molecular identification,...