CDP diacylglycerol synthase (CDS) catalyses the conversion of phosphatidic acid (PA) to CDP-diacylglycerol, an essential intermediate in the synthesis of phosphatidylglycerol, cardiolipin and phosphatidylinositol (PI). CDS activity has been identified in mitochondria and endoplasmic reticulum of mammalian cells apparently encoded by two highly-related genes, CDS1 and CDS2. Cardiolipin is exclus...

The release of carnitine palmitoyltransferase (CPT) activity from rat liver mitochondria by increasing concentrations of digitonin was studied for mitochondrial preparations from fed, 48 h-starved and diabetic animals. A bimodal release of activity was observed only for mitochondria isolated from starved and, to a lesser degree, from diabetic rats, and it appeared to result primarily from the e...

Prompted by pronounced structural differences between rat liver and rat hepatocellular carcinoma mitochondria, we suspected these mitochondrial populations to differ massively in their molecular composition. Aiming to reveal these mitochondrial differences, we came across the issue on how to normalize such comparisons and decided to focus on the absolute number of mitochondria. To this end, flu...

RTIFICIAL mixtures of mitochondria from various unrelated inbred lines A of maize exhibit oxidative and phosphorylative activities which correspond to the activities of mitochondria of hybrids of these lines ( SARKISSIAN and SRI~ASTAVA 1967; MCDANIEL and SARKISSIAN 1968). This phenomenon has been called mitochondrial complementation (MCDANIEL and SARKISSIAN 1966). Complementation reflects dissi...

Mitochondria, which play a critical role in energy production by oxidative respiration, are highly dynamic organelles and their double membranes undergo frequent events of fusion fission. Mitochondria believed to be derived from the endosymbiosis proteobacteria, thus mitochondria still contain own DNA, mitochondrial DNA (mtDNA). Several copies mtDNA form nucleoid with DNA-binding proteins. Rece...

The total activity of pyruvate dehydrogenase (PDH) complex in rat hind-limb muscle mitochondria was 76.4 units/g of mitochondrial protein. The proportion of complex in the active form was 34% (as isolated), 8-14% (incubation with respiratory substrates) and greater than 98% (incubation without respiratory substrates). Complex was also inactivated by ATP in the presence of oligomycin B and carbo...

The article presents the results of an in vitro study synergetic effect evaluation combined preparation based on coordination complex ethylmethylhydroxypyridine succinate and trimethylhydrazinium propionate energy metabolism cell respiration. aim was to evaluate mitochondria-directed action metabolic antioxidant succinic acid with relation optimizing cells under oxidative stress conditions, as ...

1. The effects of phenylpyruvate, a metabolite produced in phenylketonuria, on the pyruvate dehydrogenase-complex activity were investigated in rat brain mitochondria. 2. Pyruvate dehydrogenase activity was measured by two methods, one measuring the release of 14CO2 from [1-1"C]pyruvate and the other measuring the acetyl-CoA formed by means of the coupling enzyme, pigeon liver arylamine acetylt...

The ATPase activity of mammalian mitochondria can be readily stimulated by a variety of protonophoric uncoupling agents, the extent being dependent on the assay conditions and the isolation medium used 111. The situation in plant mitochondria is less straight-forward. Jung and Laties [2] classified plant mitochondria into three types: (i) those where uncoupler readily stimulates activity, as in...