In this study, the effects of floret sterilization with sodium hypochlorite, cold stress, heat shock, 2,4-dichlorophenoxyacetic acid and colchicine treatment on microspore viability and induction of symmetrical nuclei divisions were assessed in six genotypes of sugarcane. The highest microspore viability was observed when florets were sterilized with 3.0% and 3.5% sodium hypochlorite in all gen...

The development of the pollen grain wall in Xiphidium coeruleum (Haemodoraceae) was studied using TEM and cytochemical staining techniques. Microsporocyte ontogeny initiates with the degradation of the cellulosic cell wall and subsequent deposition of a thick callosic cell wall. Following callose deposition, successive meiosis occurs, resulting in a tetragonal tetrad of microspores. during meio...

Abstract The pollen analysis has become an important technique to evaluate the use of selectively breeding crops, including three most grasses human nutrition. This study aimed reproductive potential oat species and their hybrid progeny (amphiploids) during consecutive vegetation seasons. Correlation regression analyses were used describe morphotypes viability grains, while numerical taxonomy m...

Spores or pollen from a single species even an individual plant sporangium may vary in morphology and size to point that equivalent forms found dispersed the sediment have been described as different genera. In addition, not all these organs were mature at moment of burial, therefore they do always contain completely developed microspores. To understand variations better, we studied intraspecif...

Annonaceae, comprising approximately 107 genera and 2400 species, is the largest family among early-divergent Magnoliales. Previous studies have concentrated on binding mechanism that holds together four members of tetrads in Annonaceae. However, development mechanisms different tetrad types remain largely unknown. Mitrephora tomentosa was found to exhibit five permanent types, with two or thre...

haploid plant per floret (Devaux, 1988). Androgenesis induction in microspores may be affected by various An efficient doubled-haploid production technology that induces factors which cause low induction efficiency and by gehomozygosity can greatly reduce the time and cost of cultivar developnotype dependence (Dunwell, 1985). Most advances toment. Low efficiency of doubled-haploid production pr...

A modification of a protocol used to induce tobacco microspore embryogenesis was tested in eggplant (Solanum melongena L.). In tobacco, uninucleate microspores are subjected to stress treatment by culturing in mannitol containing “B” medium at 33°C for six days. The microspores are then transferred to maltose containing AT3 medium for further development. In the experiment presented here late u...