A modified rapid slide agglutination test for the presumptive identification of Brucella canis infection in dogs has been developed. The method required mixing 0.1 ml of canine serum with 0.1 ml of 0.2 M 2-mercaptoethanol solution. Equal volumes (0.05 ml) of the treated serum and the B. canis plate antigen were mixed. Agglutination results were read within 2 min. Clinical studies showed 100% ag...

Similar results were obtained when testing human sera for Salmonella antibodies by the tube agglutination test and by the Microtiter plate agglutination test. The plate test was easier to perform and saved time, space, antigen, and serum.

leptospirosis is a worldwide zoonosis caused by leptospira interrogans. this study was conducted toevaluate serologic and bacteriologic findings of leptospirosis in clinically-suspected cows. 380 sera and 33urine samples were collected from 6 industrial dairy farms in tehran suburb, from december 2004 to june2005. the prevalence of disease was determined by microscopic agglutination test (mat),...

A new latex agglutination test for rubella virus was used to test sera with a hemagglutination inhibition titer of -8 (97 specimens) or >256 (158 specimens). Maximum latex agglutination test sensitivity was achieved when low-titer sera were tested undiluted and high-titer sera were diluted (1:10). A modification of the protocol of the manufacturer resulted in a latex agglutination-hemagglutinat...

Measurement of von Willebrand factor antigen (VWF : Ag) levels is usually performed in a specialised laboratory which limits its application in routine clinical practice. So far, no commercial rapid test kit is available for VWF : Ag estimation. This paper discusses the technical aspect of latex agglutination method which was established to suit the purpose of estimating von Willebrand factor (...

Three commercially available rapid slide agglutination tests for the identification of Staphylococcus aureus were evaluated with 354 recent clinical isolates (165 strains of S. aureus). The test results of two latex agglutination products, SeroSTAT Staph (Scott Laboratories, Inc.) and Staphylatex (American Micro Scan), and one hemagglutination product, Staphyloslide (BBL Microbiology Systems), ...

Leptospirosis is a zoonosis of worldwide distribution, caused by Leptospira interrogans. It is a well known cause of bovine reproductive losses such as abortion, infertility, stillbirth and birth of weak calves. Inthis research, the relationship between the seroprevalence rate of Leptospira spp. infection and abortion inindustrial dairy farms of Hamedan province, Iran was studied. A total of 80...

two hundred and forty patients, who had undergone vasectomy from 1977 to 1985 and subsequent vasovasostomy ,were studied for the presence of sperm-specific antibodies by using the kibrick's gelatin agglutination test. the number of successful pregnancies and the presence of agglutination were also considered in this survey. sixty-nine pregnancies occurred in total and agglutination was present ...

INTRODUCTION Norway rats (Rattus norvegicus) are zoonotic reservoirs for Leptospira spp. and Toxoplasma gondii, and influence diseases in urban areas. METHODS Free-ranging and laboratory-raised rats from two zoos in southern Brazil were tested for Leptospira spp. and T. gondii using microscopic agglutination and modified agglutination tests, respectively. RESULTS Overall, 25.6% and 4.6% fre...