objective: the aim of the present study was the production of recombinant lentviruses that express mir-16. after transduction, altered expression levels of mirna and its target protein were analyzed. methods: a dna fragment that contained the mir-16 precursor was cloned in a lentiviral plasmid. lentiviral vector particles were produced by transient calcium phosphate co-transfection of 293t cell...

WAC 173-205-010 Purpose. 173-205-020 Definitions. 173-205-030 Applicability. 173-205-040 Determining the need for effluent characterization. 173-205-050 Effluent testing for toxicity. 173-205-060 Additional effluent characterizations. 173-205-070 Monitoring for compliance with whole effluent toxicity limits. 173-205-080 Samples for whole effluent toxicity testing. 173-205-090 Response to noncom...

Esophageal cancer is a common cause of cancer death worldwide. Esophageal squamous cell carcinoma (ESCC) is the most predominant type. Certain microRNAs (miRNAs) function in tumorgenesis involved in important biological and pathologic processes. To reveal miRNAs’ signatures of ESCC, we analyzed miRNAs extracted from ESCC cell lines with the microRNA microarray. The significant alterations were ...

To investigate the mechanism of lidocaine in inhibiting proliferation, migration and invasion breast cancer cells. Human cell line Michigan Cancer Foundation-7 cells were treated with lidocaine. Cell proliferation was detected by 2,5-diphenyl-2H-tetrazolium bromide, Transwell, microRNA-495-3p expression quantitative reverse transcription polymerase chain reaction fibroblast growth factor 9, cyc...

Esophageal cancer is a common cause of cancer death worldwide. Esophageal squamous cell carcinoma (ESCC) is the most predominant type. Certain microRNAs (miRNAs) function in tumorgenesis involved in important biological and pathologic processes. To reveal miRNAs’ signatures of ESCC, we analyzed miRNAs extracted from ESCC cell lines with the microRNA microarray. The significant alterations were ...

The enzyme 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2) is selectively expressed in aldosterone target tissues, conferring aldosterone selectivity for the mineralocorticoid receptor. A diminished activity causes salt-sensitive hypertension. The mechanism of the variable and distinct 11β-hydroxysteroid dehydrogenase type 2 gene (HSD11B2) expression in the cortical collecting duct is poorly...

To explore the function of kaempferol on lung cancer cell A549 and microRNA-199 variation is objective study. The proliferation was detected by counting kit-8. expression mammalian target rapamycin, phosphorylated light chain 3-?/?, beclin-1, B-cell lymphoma 2 2-associated X protein showed Western blot. determined quantitative polymerase reaction. adenocarcinomic human alveolar basal epithelial...