BACKGROUND Shotgun metagenomics has become an important tool for investigating the ecology of microorganisms. Underlying these investigations is the assumption that metagenome sequence data accurately estimates the census of microbial populations. Multiple displacement amplification (MDA) of microbial community DNA is often used in cases where it is difficult to obtain enough DNA for sequencing...

Microbial population growth is typically measured when cells can be directly observed, or when death is rare. However, neither of these conditions hold for the mammalian gut microbiota, and, therefore, standard approaches cannot accurately measure the growth dynamics of this community. Here we introduce a new method (distributed cell division counting, DCDC) that uses the accurate segregation a...

The growth of watermelon is often threatened by Fusarium oxysporum f. sp. niveum (Fon) in successively monocultured soil, which results in economic loss. The objective of this study was to investigate the effect of D123 wheat as a companion crop on soil enzyme activities, microbial biomass and microbial communities in the rhizosphere of watermelon and to explore the relationship between the eff...

The effect of UMMB supplementation on rumen micro ora was investigated in  fteen healthy rumen  stulated buffalo calves divided into three groups having  ve animals in each. The animals of group I, II and III were kept on conventional diet, wheat straw alone and wheat straw supplemented with UMMB, respectively. Rumen liquor samples were collected at different time intervals for three consec...

Soil microbes are involved in many important ecosystem processes including nutrient acquisition, biogeochemical cycling and soil aggregation. microbial diversity affects the belowground dynamics fate of carbon nutrients. for agricultural plant production systems, hence understanding effects agroforestry systems on microbes, is necessary order to improve health fertility. The objective study was...

Shredded carrots in modified atmosphere packaging were treated with low-dose irradiation of 0.5 kGy in order to determine whether additional reduction of microbial population would be achieved for carrots previously treated with chlorine. Commercially prepared shredded carrots treated with irradiation had a mean microbial population of 1300 CFU/g at the expiration date (9 days after irradiation...

The gastrointestinal microbiota of neonatal pigs may be affected by dam parity. Summary Litter performance, progeny growth performance, and progeny health status may be affected by dam parity. The objective of the current experiment was to evaluate gastrointestinal microfl ora, as a measure of gut health, in progeny derived from fi rst parity (P1) compared to fourth parity (P4) dams. Fecal samp...

Extensive fine-scale genetic diversity is found in many microbial species across varied environments, but for most, the evolutionary scenarios that generate the observed variation remain unclear. Deep sequencing of a thermophilic cyanobacterial population and analysis of the statistics of synonymous single-nucleotide polymorphisms revealed a high rate of homologous recombination and departures ...

The effects of Selko-pH (S; 1 mL/L drinking water); Termin-8 (T; 2 kg/ton feed) and Neogermicin (N; 2 L/ton feed) on gastrointestinal tract (GIT) pH, microbial population, ileal digestibility and broiler performance were investigated. The lowest feed intake (FI) was attained by chickens fed diets supplemented with S group in all periods (P

Introduction: In this study, the chemical composition and antimicrobial activity of essential oil of Ziziphora clinopodiodes and its potential application as a natural preservative in reducing the indigenous microbial population of hamburger were investigated. Method: Essential oil of Ziziphora clinopodiodes cultivated in Iran was obtained by Hydro-distillation method (HDM). Chemical compositio...