We present a technique for the fast screening of the lead concentration in whole blood samples using laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS). The whole blood sample is deposited on a polymeric surface and wiped across a set of micro-grooves previously engraved into the surface. The engraving of the micro-grooves was accomplished with the same laser system used fo...

The cellular basis of the cytolytic cross-reactivity observed in primary allogeneic (C56BL/6 anti-DBA/2 and C57BL/6 anti-C3H/He) mixed-leukocyte cultures (MLC) was investigated by analysis of the specificity of clonal progeny derived from individual cytolytic T lymphocyte (CTL) precursor cells (CTL-P) contained within these populations. A sensitive mixed-leukocyte microculture (micro-MLC) techn...

SM-197436, SM-232721, and SM-232724 are new 1beta-methylcarbapenems with a unique 4-substituted thiazol-2-ylthio moiety at the C-2 side chain. In agar dilution susceptibility testing these novel carbapenems were active against methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus epidermidis (MRSE) with a MIC(90) of </=4 micro g/ml. Furthermore, SM-232724 showed strong bacterici...

For adaptation between anaerobic, micro-aerobic and aerobic conditions Escherichia coli's metabolism and in particular its electron transport chain (ETC) is highly regulated. Although it is known that the global transcriptional regulators FNR and ArcA are involved in oxygen response it is unclear how they interplay in the regulation of ETC enzymes under micro-aerobic chemostat conditions. Also,...

Introduction: β2-microglobulin and prolactin are medium-molecular-weight toxins. We believe that the rate of removal these molecules, using filters with two different surfaces, in post- pre-dilution haemodiafiltration, will be interest to literature. Methods: studied, 12 haemodialyzed patients, those, filter surface 2.5 m2 (Group A) or 2.1 B) post-dilution a C). Results: Satisfactory (best post...

A simplified method has been developed for agar dilution antimicrobial susceptibility testing of anaerobic bacteria, designed to economize on time and money when only a few isolates need to be tested. The procedure is based on the principle of using filter paper disks as carriers of the antibiotic and 35- by 10-mm petri dishes which, when inoculated with the Steers replicator, can test up to fo...

Abstract This work aimed to study the antimicrobial activity of eight various components plant origin on growth Pseudomonas lundensis and Listeria monocytogenes. Different in vitro methods were used: agar plate diffusion, micro atmosphere, hole micro-dilution, gradient-plate method. In first assay, p-cymene γ-terpinene did not inhibit tested bacteria therefore they used further experiments. Bot...

because of its kinetic nature and computational advantages, the lattice boltzmann method (lbm) has been well accepted as a useful tool to simulate micro-scale flows. the slip boundary model plays a crucial role in the accuracy of solutions for micro-channel flow simulations. the most used slip boundary condition is the maxwell slip model. the results of maxwell slip model are affected by the ac...

As a fundamental study relevant to micro-combustor application, the effects of mixture dilution on the lean extinction characteristics is studied for a stagnation-point flow system with a methane/air mixture over a platinum surface. For steady conditions, the level of flammability extension by surface reaction depends strongly on the mixture dilution, such that the benefit of catalyst-assisted ...