The hydrochemical analysis method was used to reveal the sources and spatiotemporal variations of carbon nitrogen elements in Pingzhai Reservoir, C–N coupling cycle its influence on karst sink are discussed. results show following: (1) type study area is HCO3-Ca. (2) From river reservoir then outlet, values HCO3− δ13CDIC showed an opposite trend. NO3−, δ15N-NO3−, δ18O-NO3− were different each s...

Yeast phenylalanine tRNA (tRNAPhe) exhibits a 4$-fold enhancement of fluorescence in response to the addition of Mg2+. Concurrently, over the same concentration range (0 to 0.4 m&r) of Mg2+, the circular dichroism peak at 263 nm displays a 20% increase in ellipticity and a blue shift of 2 nm. The existence of the negative circular dichroism band at 295 nm appears to be cation-dependent. This co...

Yeast phenylalanine tRNA (tRNAPhe) exhibits a 4$-fold enhancement of fluorescence in response to the addition of Mg2+. Concurrently, over the same concentration range (0 to 0.4 m&r) of Mg2+, the circular dichroism peak at 263 nm displays a 20% increase in ellipticity and a blue shift of 2 nm. The existence of the negative circular dichroism band at 295 nm appears to be cation-dependent. This co...

to membranes in brain and liver was similar to, but more marked than, that observed with Mg2.+ (Table 2). This can be explained on the basis of a possible interaction between cations and the negatively charged groups on the endoplasmic-reticulum membranes. Brain endoplasmic reticulum has considerably more phospholipid than liver, hence the requirement for spermine and Mg2+ may also be greater i...

Cost-effective and controllable synthesis of M0.25Ni0.15Cu0.25Co0.35Fe2O4 (M2+ = Mg2+, Mn2+, Cd2+) ferrites via the sol–gel auto-combustion technique. The impact divalent cations on structural, dielectric, optoelectrical properties was examined by XRD, FTIR, Raman, LCR, UV–Vis, two probe I-V measurement techniques. crystallite size 52.66 nm, minimum specific surface area observed 5.1507 m2/g fo...

The binding of Mg2+ to the ribitol teichoic acid of Staphylococcus aureus H walls was examined by equilibrium dialysis in solution and in the intact wall; the influence of alanyl ester groups on binding was determined. In solution the ribitol polymer had a lower affinity than did a glycerol teichoic acid and bound Mg2+ in the ratio Mg2+/P of 1:1. The presence of alanyl ester residues caused a d...

The binding of cations to ATP-G-actin has been assessed by measuring the kinetics of the increase in fluorescence of N-acetyl-N'-(5-sulfo-1-naphthyl)-ethylenediamine-labeled actin. Ca2+ and Mg2+ compete for a single high-affinity site on ATP-G-actin with KD values of 1.5-15 nM for Ca2+ and 0.1-1 microM for Mg2+, i.e. with affinities 3-4 orders of magnitude higher than previously reported (Fried...

Intracellular levels of the divalent cations Ca2+ and Mg2+ are important regulators of cell cycle and proliferation. However, the precise mechanisms by which they are regulated in cancer remain incompletely understood. The channel kinases TRPM6 and TRPM7 are gatekeepers of human Ca2+/Mg2+ metabolism. Here, we investigated the human neuroblastoma cell line SHEP-21N in which the MYCN oncogene (en...

The paper presents the results of physicochemical analyses spring waters in Postomia River valley (Northwest Poland). Multivariate statistical methods, i.e., cluster analysis (CA) and principal component (PCA) were used to assess spatial distribution similarities differences concentrations individual elements. Concentrations macro elements (MEs), trace (TEs) rare-earth (REEs) analysed concernin...

Drosophila mutants provide an ideal model to study channel-type specificity of ion channel regulation in situ. In this study, the effects of divalent cations on voltage-gated K+ currents were investigated in acutely dissociated central neurons of Drosophila third instar larvae using the whole-cell patch-clamp recording. Our data showed that micromolar Cd2+ enhanced the peak inactivating current...