To make low-cost catalytic materials that mimic the activity of tyrosinase enzymes 29 (Catechol oxidase) is an exciting challenge of biochemical technology. Herein, we report the 30 synthesis of a series of acylhydrazone-pyrazoles based biomolecule materials (L1-L7) with superior 31 catecholase activity. These biomolecules were synthesized by a one pot chemical condensation 32 between 5-methyl-...

and epinephrine) play important roles not only in the peripheral nervous system but in the central one. One of the inactivation pathways of CAs is the enzymatic metabolism by catechol-O-methyltransferase (COMT; EC2.1.1.6), which methylates their catechol moieties using S-adenosyl-L-methionine (SAMe) as a methyl donor. There are two COMT isoforms: in the cytoplasm as soluble COMT (S-COMT) and in...

This study was specifically designed to identify anticancer constituents in methanol-water extract of Polygonum bistorta L. and evaluate its cytotoxicity. For this purpose methanol-water (40:60 v/v) extract was subjected to conventional preparative high pressure liquid chromatography and 13 fractions were obtained. Constituents of obtained fractions were separated and identified with the help o...

The inhibitory effect of nordihydroguaiaretic acid (NDGA) (a nonselective lipoxygenase (LOX) inhibitor)-mediated 15-LOX inhibition has been reported to be affected by modification of its catechol ring, such as methylation of the hydroxyl group. Cannabidiol (CBD), one of the major components of marijuana, is known to inhibit LOX activity. Based on the phenomenon observed in NDGA, we investigated...

Cupriavidus necator JMP134 is a model for chloroaromatics biodegradation, capable of mineralizing 2,4-D, halobenzoates, chlorophenols and nitrophenols, among other aromatic compounds. We performed the metabolic reconstruction of aromatics degradation, linking the catabolic abilities predicted in silico from the complete genome sequence with the range of compounds that support growth of this bac...

It has long been recognized that lignin is a potential renewable source of chemicals, however, there has been only limited success in the commercialization of processes for converting lignin into chemicals [1,2]. Alkaline hydrolysis of lignin has received considerable attention because the reagents are relatively inexpensive and the phenolic products obtained are of potential value [1]. The mos...

Transient A-type K+ channels (I(A)) in neurons have been implicated in the delay of the spike onset and the decrease in the firing frequency. Here we have characterized biophysically and pharmacologically an I(A) current in lamprey locomotor network neurons that is activated by suprathreshold depolarization and is specifically blocked by catechol at 100 microM. The biophysical properties of thi...

Prion proteins are considered as the main agents for the Transmissible Spongiform Encephalopathies (TSE). The misfolded form, PrP Sc , which is also indicated as the etiological agent for TSE, exhibits high resistance to degradation in environmental processes. Soil contamination by prion proteins is a real environmental issue since contaminated soils can become potential reservoir and diffuser ...

An inexpensive, disposable microfluidic device was fabricated from a dry film photoresist using a combination of photolithographic and hot roll lamination techniques. A microfluidic flow pattern was prefabricated in a dry film photoresist tape using traditional photolithographic methods. This tape became bonded to a poly(methyl methacrylate) (PMMA) sheet with prepouched holes when passed throug...

High-throughput screening led to the identification of a 3-norbornyl derivative of catechol called 48F10 (3-bicyclo[2.2.1]hept-2-yl-benzene-1,2-diol) as a Kv2.1 K(+) channel inhibitor. By virtue of the involvement of Kv2.1 channels in programmed cell death, 48F10 prevents apoptosis in cortical neurons and enterocytes. This uncharged compound acts with an apparent affinity of 1 muM at the tetrae...