Metallo beta lactamases (MBL) are enzymes that have wide spread of activity and they confer a high level of resistance to all b-lactams including carbapenem, except aztreonam [1]. MBLs require divalent zinc ion for their enzymatic activity which is not diminished by serine b lactamase inhibitors like salbactam, tazobactam, clavulinic acid etc but is inhibited by metal chelators like EDTA and th...

background and objectives : carbapenem resistant pseudomonas aeruginosa is a serious cause of nosocomial infections.the main purpose of the study is to determine the prevalence rate of imipenem resistant pseudomonas aeruginosa carrying metallo- beta- lactamase (mbl) genes. material and methods : 236 pseudomonas aeruginosa isolates were collected from teaching hospitals of ahvaz university of me...

One of the urgent problems modern health care is growing resistance microorganisms to antibiotics, including carbapenems, which until recently were considered as drugs choice in treatment life-threatening infections. Enzymatic inactivation through production carbapenemase, main mechanism Gram-negative bacteria. The these infections presents significant difficulties due extremely limited arsenal...

BACKGROUND The beta-lactamase landscape is changing radically, with CTX-M types now the most prevalent extended-spectrum beta-lactamases (ESBLs) worldwide, except maybe in the USA. In addition, there are growing numbers of Enterobacteriaceae with KPC and metallo-carbapenemases. We examined whether combinations of oxyimino-cephalosporins with NXL104, a novel non-beta-lactam beta-lactamase inhibi...

PURPOSE The present study was undertaken to detect metallo-beta-lactamase (MBL) in nosocomial isolates of Pseudomonas aeruginosa by four different phenotypic methods. METHODS Ninety-one consecutive P. aeruginosa isolates were subjected to susceptibility testing by disc-diffusion assay and Vitek 2. Imipenem resistance was determined by three different methods (disc-diffusion, Vitek 2 and E tes...

Introduction: Metallo-beta-lactamase (MBL)-producing Acinetobacter is a therapeutic challenege all over the world. The aims of this study were to determine the prevalence of MBL genes and evaluate the antimicrobial susceptibility profile of carbapenem non-susceptible isolates of Acinetobacter. Material and Method: During a period of 12 months (November 2012 to October 2013) 94 isolates of carba...

Acinetobacter baumanii has emerged as an important opportunistic pathogen within the hospital environment, being able to colonize and produce infections in ventilator associated pneumonia, secondary meningitis, urinary tract, septicaemia and other conditions in intensive care unit (ICU) patients. It is also causing infections in other immunocompromised patients, including burns patients. Antimi...

The gene encoding the metallo-beta-lactamase SPM-1 was cloned from a genomic library of Pseudomonas aeruginosa strain 48-1997 A. The insert carrying spm-1 possessed a GC content of 47%, indicating that it is of non-Pseudomonas origin. Upstream of spm-1 there is a small open reading frame (ORF), which is homologous to the LysR family of proteins (69% identity to the LysR protein from Salmonella ...

In eukaryotes, the process of messenger RNA 3'-end formation involves endonucleolytic cleavage of the transcript followed by synthesis of the poly(A) tail. The complex machinery involved in this maturation process contains two proteins of the metallo-beta-lactamase (MBL) superfamily, the 73 and 100 kDa subunits of the cleavage and polyadenylation specificity factor (CPSF). By using an in vitro ...

BACKGROUND Pseudomonas aeruginosa (PA) has a non-clonal, epidemic population with a few widely distributed and frequently encountered sequence types (STs) called 'high-risk clusters'. Clinical P. aeruginosa (clinPA) has been studied in all inhabited continents excepted in Africa, where a very few isolates have been analyzed. Here, we characterized a collection of clinPA isolates from four count...