background: pseudomonas aeruginosa is the causing agent of many hospital infections and metallo-beta-lactamases (mbl) are being reported with increasing frequency. the aim of this study was to determine the frequency of metallo-&beta-lactamases; (mbl) and vim-1 gene in multidrug-resistant strains of p. aeruginosa isolates and to compare the methods of phenotypic and molecular detection. materia...

Background: A prospective study was undertaken to detect metallo-β-lactamases (MBLs) and AmpC β-lactamases in Pseudomonas aeruginosa isolates recovered from patients at Indira Gandhi Medical College, Shimla (H.P). Objective: The study aimed at determining the prevalence of MBL and AmpC producing P. aeruginosa isolates. Material and Methods: A total of 180 isolates were preliminarily screened fo...

During the last 30 years there has been a dissemination of plasmid-mediated β-lactamases in Enterobacteriaceae in Brazil. Extended spectrum β-lactamases (ESBL) are widely disseminated in the hospital setting and are detected in a lower frequency in the community setting. Cefotaximases are the most frequently detected ESBL type and Klebsiella pneumoniae is the predominant species among ESBL prod...

BACKGROUND The emergence of carbapenem-resistant Acinetobacter baumannii (CRAB) was observed in a Kenyan hospital from 2009 to 2010. Further investigation of the dissemination of CRAB isolates and the molecular characterization of associated resistance determinants were therefore performed. METHODS Antibiotic susceptibilities were determined by broth microdilution and Etest. Metallo-β-lactama...

New Delhi metallo-β-lactamase-1 (NDM-1) has attracted extensive attention for its high catalytic activities of hydrolyzing almost all β-lactam antibiotics. NDM-1 shows relatively higher similarity to subclass B1 metallo-β-lactamases (MβLs), but its residue at position 229 is identical to that of B2/B3 MβLs, which is a Tyr instead of a B1-MβL-conserved Trp. To elucidate the possible role of Y229...

Metallo-β-lactamases (MBLs) are of increasing clinical significance; the development of clinically useful MBL inhibitors is challenged by the rapid evolution of variant MBLs. The Verona integron-borne metallo-β-lactamase (VIM) enzymes are among the most widely distributed MBLs, with >40 VIM variants having been reported. We report on the crystallographic analysis of VIM-5 and comparison of bioc...

background :     river and underground waters are main sources of tap water in guilan, iran. overland wastes move into rivers during periods of heavy or extended rain that is very common in the area. pseudomonas aeruginosa , legionella pneumophila , and escherichia coli are main human pathogens with water source. this study is designed to determine the load of these bacteria in main water suppl...

Elizabethkingia meningoseptica is an emerging, healthcare-associated pathogen causing a high mortality rate in immunocompromised patients. We report the draft genome sequence of E. meningoseptica Em3, isolated from sputum from a patient with multiple underlying diseases. The genome has a length of 4,037,922 bp, a GC-content 36.4%, and 3673 predicted protein-coding sequences. Average nucleotide ...

INTRODUCTION: In this study, we used phenotypic methods to screen carbapenem-resistant Enterobacteriaceae (CREs) and evaluated their antimicrobial sensitivity profile. METHODS: One hundred and seventy-eight CREs were isolated at a university hospital in south Brazil in a one-year period. Samples were assessed using disk diffusion tests with inhibitors of β-lactamases such as phenylboronic ac...