Luzzati, Denise (Institut de Biologie Physico-Chimique, Paris, France). Effect of thymine starvation on messenger ribonucleic acid synthesis in Escherichia coli. J. Bacteriol. 92:1435-1446. 1966.-During the course of thymine starvation, the rate of synthesis of messenger ribonucleic acid (mRNA, the rapidly labeled fraction of the RNA which decays in the presence of dinitrophenol or which hybrid...

Messenger RNA maturation in eukaryotes typically involves the removal of introns from long precursor molecules. An unusual form of RNA splicing in which separate precursor transcripts contribute sequences to the mature mRNA through intermolecular reactions has now been documented in a number of diverse organisms. In this review, the phenomenon of pre-mRNA trans-splicing has been divided into tw...

In eukaryotes, messenger RNAs are generated by a process that includes coordinated splicing and 3 9 end formation. Factors essential for the splicing of mRNA precursors (pre-mRNA) 1 in eukaryotes have been identified primarily through the study of nuclear extracts derived from mammalian cells and Saccharomyces cerevisiae genetics. Here, we identify homologues of most known pre-mRNA processing f...

Glucose homeostasis is strictly controlled in all domains of life. Bacteria that are unable to balance intracellular sugar levels and deal with potentially toxic phosphosugars cease growth and risk being outcompeted. Here, we identify the conserved haloacid dehalogenase (HAD)-like enzyme YigL as the previously hypothesized phosphatase for detoxification of phosphosugars and reveal that its synt...

The flow of genetic information is regulated by selective nucleocytoplasmic transport of messenger RNA:protein complexes (mRNPs) through the nuclear pore complexes (NPCs) of eukaryotic cells. However, the three-dimensional (3D) pathway taken by mRNPs as they transit through the NPC, and the kinetics and selectivity of transport, remain obscure. Here we employ single-molecule fluorescence micros...

Abstract Background Maternal vaccination for Influenza and Tetanus, Diphtheria, acellular Pertussis (TDaP) have been well studied in terms of safety efficacy protection the newborn by placental passage antibodies. Similar would be expected after maternal against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), virus responsible disease 2019 (COVID-19). There is a significant urgent...

An RNA fraction with messenger activity has been isolated from free polysomes of rabbit liver. This liver RNA was translated in a reticulocyte cell-free system, which had been rendered dependent on exogenous messenger RNA by mild ribonuclease treatment. After ribonuclease was removed from the ribosomes, protein synthesis was almost totally dependent on 0.5 M KC1 ribosomal wash protein and exoge...

Protein synthesis in all cells begins with the ordered binding of the small ribosomal subunit to messenger RNA (mRNA) and transfer RNA (tRNA). In eukaryotes, translation initiation factor 3 (eIF3) is thought to play an essential role in this process by influencing mRNA and tRNA binding through indirect interactions on the backside of the 40S subunit. Here we show by directed hydroxyl radical pr...

Background The registration trials of messenger RNA (mRNA) vaccines against SARS-CoV-2 did not address patients with inflammatory rheumatic diseases (IRD). Objective To assess the humoral response after two doses mRNA vaccine SARS-CoV-2, in IRD treated immunomodulating drugs and impact on activity. Methods Consecutive at rheumatology institute, who received their first (Pfizer) vaccine, were re...

The presence of angiotensinogen messenger RNA (mRNA) was assessed in total RNA extracted from hepatoma, glioma, neuroblastoma, and glioma-neuroblastoma hybrid cell lines. Total RNA from 1 X 10(7) cells was extracted, transferred to a membrane, and hybridized with a 32P-labeled, full-length (1650-base pair) rat angiotensinogen complementary DNA (cDNA). Angiotensinogen RNA sequences could be defi...