Understanding the kinetic mechanism of enzyme inactivation by suicide substrate is relevance for optimal design new drugs with pharmacological and therapeutic applications. Suicide usually occurs via a two-step mechanism, although there are enzymes such as peroxidase catalase in which H2O2 happens single step. The approximate solution ordinary differential equation (ODE) system one step kinetic...

To understand the behaviour of complex systems, it is often necessary to use models that describe the dynamics of subnetworks. It has previously been established using projection methods that such subnetwork dynamics generically involves memory of the past and that the memory functions can be calculated explicitly for biochemical reaction networks made up of unary and binary reactions. However,...

Chemical reactions inside cells occur in compartment volumes in the range of atto- to femtoliters. Physiological concentrations realized in such small volumes imply low copy numbers of interacting molecules with the consequence of considerable fluctuations in the concentrations. In contrast, rate equation models are based on the implicit assumption of infinitely large numbers of interacting mol...

The quasi-steady-state approximation (QSSA) is a model reduction technique used to remove highly reactive species from deterministic models of reaction mechanisms. In many reaction networks the highly reactive intermediates (QSSA species) have populations small enough to require a stochastic representation. In this work we apply singular perturbation analysis to remove the QSSA species from the...

dose-dependent pharmacokinetic of phenytoin necessitates the estimation of the maximum rate of metabolism (vm) and the michaelis-menten constant (km) in a concerned population. the aim of this study was to determine the pharmacokinetic parameters of phenytoin in a sample of iranian patients to optimize the antiepileptic pharmacotherapy. fourty patients who received a constant dose of phenytoin ...

0.1016/j.pisc.2014 he Author. Publish mons.org/licenses t of an special issu etics”. Copyright thor. [email protected] Abstract The year 2013 marked the centenary of the paper of Leonor Michaelis and Maud Menten (Michaelis and Menten, 1913), and the 110th anniversary of the doctoral thesis of Victor Henri (Henri, 1903). These publications have had an enormous influence on the progress of biochem...