interspecific or intergenus hybridization in sugarbeet (b. vulgaris l.) is usually failed due to non fertilization, embryo death or absence of connection between embryo and endosperm. consequently invitro embryo rescue technique is of value to save hybrid embryos before annihilation. sugar beet inter-specific crosses with wild species is impossible in classical breeding because of no chromosoma...

Background: Studies of preimplantation stage embryos by classic cytogenetic techniques have limitations, starting with the need for good metaphase stage when only one third of all analyzed embryos may show good quality metaphases. The incidence of chromosome anomalies in embryos produced by in vitro procedures is generally higher than that of embryos formed in vivo. Pressure specifically affect...

Prolonged in vitro cultivation allows us to obtain better quality embryos and provides better intrauterine conditions for transfer. The aim of this study was to compare the results of in vitro fertilisation (IVF) procedures in which 1, 2 or 3 blastocysts (group 1) were transferred with the results of IVF procedures in which 1, 2 or 3 embryos at the stage of 8 cells to morula, achieved under the...

BACKGROUND Assisted hatching (AH) is a manipulation of zona pellucida aiming to facilitate embryo implantation. METHODS Systematic review and meta-analysis of medical literature was used to evaluate the effect of AH on assisted reproduction outcomes: clinical pregnancy, live birth, multiple pregnancy and miscarriage. Additional analysis was performed in these subgroups: (i) fresh embryos tran...

The growth and development of three groups of genetically identical F1 C57BL/6J female x SJL/J male mice were compared to examine whether embryo manipulation affects subsequent postnatal growth and development of mammalian embryos: (1) controls--the natural offspring of timed matings, (2) transferred controls--offspring from 2-cell embryos transferred to recipients 1 day asynchronous, and (3) t...

Protocols for the production and transformation of grapevine embryogenic cultures are described. Embryogenic cultures are initiated from leaves or stamens and pistils and transformed with Agrobacterium containing an enhanced green fluorescent protein/neomycin phosphotransferase II (egfp/nptII) fusion gene. Cultures are transferred to induction medium in the dark for callus formation and prolife...

BACKGROUND Characteristics routinely used to evaluate embryo quality after thawing include number of blastomeres survived and presence of mitosis resumption after overnight culture. It is unknown to which extent symmetry and fragmentation affect implantation after warming and whether application of stricter criteria either before vitrification or after warming would improve implantation rate (I...

OBJECTIVE Great advances have been made in the field of assisted reproductive technology (ART) since the first in vitro fertilization (IVF) baby was born in Korea. This study was designed to report on the current status of ART therapy in South Korea between January 1 and December 31 of 2010. METHODS A revised survey, originally developed by the International Committee Monitoring Assisted Repr...