Enzymes are commonly defined as biological catalysts, regulating particular biochemical reactions. α-Amylase (EC 3.2.1.1) is one of the industrially important enzymes, which extensively used in starch hydrolyzing processes, such brewing, fermentation, detergent production, food processing, etc. This enzyme breaks down α-1,4 glycosidic bonds amylose or amylopectin. The end products from maltotri...

This report concerns the differential utilization of the disaccharide maltose and of its constituent monosaccharide glucose by a strain of Neisseria meningitidis. In the presence of maltose the organism produced changes which in the presence of glucose either were not detected or appeared at a greatly reduced rate. For example, it was noted that inorganic phosphate disappeared only with maltose...

Corynebacterium glutamicum transiently accumulates large amounts of glycogen, when cultivated on glucose and other sugars as a source of carbon and energy. Apart from the debranching enzyme GlgX, which is required for the formation of maltodextrins from glycogen, alpha-glucan phosphorylases were assumed to be involved in glycogen degradation, forming alpha-glucose 1-phosphate from glycogen and ...

We have identified a cluster of six genes involved in trehalose transport and utilization (thu) in Sinorhizobium meliloti. Four of these genes, thuE, -F, -G, and -K, were found to encode components of a binding protein-dependent trehalose/maltose/sucrose ABC transporter. Their deduced gene products comprise a trehalose/maltose-binding protein (ThuE), two integral membrane proteins (ThuF and Thu...

monascus purpureus is a fungus that had been cultured on the rice in eastern asian countries since thousands years ago and used as a food for long years. the fungus produces very valuable metabolites with polyketide structure. the most important metabolite is monacolin k, lovastatin or competitive inhibitor of 3-hydroxy-3-methylglutaryl-coenzyme a reductase (an affective enzyme in cholesterol s...

Protein folding and unfolding are complex phenomena, and it is accepted that multidomain proteins generally follow multiple pathways. Maltose-binding protein (MBP) is a large (a two-domain, 370-amino acid residue) bacterial periplasmic protein involved in maltose uptake. Despite the large size, it has been shown to exhibit an apparent two-state equilibrium unfolding in bulk experiments. Single-...

Abstract The efficacy of alcohol/sugar aqueous biphasic (ABS) system on protein extraction was investigated. A model protein, bovine serum albumin (BSA), adopted to evaluate the effects types and concentration phase-forming components, concentration, pH partition efficiency. 1-propanol/maltose ABS exhibited an overall better efficiency BSA alcohol-rich top phase. maximum coefficient ( K ) 20.01...

We present a computational study on the driven transport of the Maltose Binding Protein (MBP) across nanochannels in the framework of coarse-grained modeling. The work is motivated by recent experiments on voltage-driven transport of MBP across nanopores exploring the influence of denaturation on translocation pathways. Our simplified approach allows a statistical mechanical interpretation of t...

The ability to regulate cellular protein activity offers a broad range of biotechnological and biomedical applications. Such protein regulation can be achieved by modulating the specific protein activity or through processes that regulate the amount of protein in the cell. We have previously demonstrated that the nonhomologous recombination of the genes encoding maltose binding protein (MBP) an...