BACKGROUND The digestion of dietary starch in humans is initiated by salivary α-amylase, an endo-enzyme that hydrolyzes starch into maltose, maltotriose and larger oligosaccharides. Salivary amylase accounts for 40 to 50% of protein in human saliva and rapidly alters the physical properties of starch. Importantly, the quantity and enzymatic activity of salivary amylase show significant individu...

Background & Aim: Singhnad Guggulu is a traditional Ayurvedic formula for detoxifying and rejuvenating the joints. The present study was to investigate the α-amylase inhibition and antioxidant activities of aqueous extract of Singhnad Guggul (AESG). Experimental: α-Amylase inhibition assay by dinitro-salicylic acid method and antioxidant activity by ferric chelating and ferrous reducing assay....

Purification of alpha-amylase from the cultivation supernatant of recombinant Bacillus subtilis by high-speed counter-current chromatography (HSCCC) in polyethylene glycol (PEG) 4000-inorganic salt aqueous polymer two-phase systems was studied. The effects of sodium chloride concentration on the partition coefficients of alpha-amylase and total protein were respectively tested in PEG4000-phosph...

To evaluate a new method for measuring pancreatic amylase in serum, in which the salivary isoenzyme is inhibited with a specific monoclonal antibody, we determined the activity of pancreatic and salivary amylase in sera from 103 healthy subjects and from 114 hospitalized patients having a wide range of total amylase activities. CVs for the proposed method ranged from 0.8% to 5.1% (within day) a...

Rabbit antiserum against beta-amylase isolated from germinating seeds of rice was produced, and its specific cross-reactivity with beta-amylase was confirmed by means of Ouchterlony double immunodiffusion and immunoelectrophoresis procedures. The cellular localization of beta-amylase was studied by indirect fluorescence microscopy of thin sectioned germinating rice seed specimens (1-day stage) ...

Muffin et al. (1), in this issue, describe a more specific method for pancreatic ainylase in serum. Amylase of salivary origin is precipitated with a monoclonal antibody, and the amylase remaining is presumed to come from the pancreas. It is likely that their test will be more specific for pancreatic disease. Since the recognition of multiple sources of antylase and of the frequency of hyperamy...

I describe a new kinetic enzymatic saccharogenic method for assaying alpha-amylase in human serum and urine. alpha-Amylase liberates maltose from starch. This is successively acted on by alpha-glucosidase, mutarotase, and glucose dehydrogenase. The resulting conversion of NAD+ to NADH, measured at 340 nm, during a 20-min incubation reflects amylase activity. Endogenous glucose is destroyed befo...

besides the high starch content, sweet potato contains endogenous amylases where the two predominants are - and β-amylases. sweet potato is thought to be a promising source of β -amylase since β -amylase is one of the major protein in the tubers. this investigation has focused on the properties of β amylase from white-flesh sweet potato that has been grown in iran as a potential source for indu...

Amylases are among the most important enzymes and are of great significance in present day industry. Starch degrading bacteria are most important for industries such as food, fermentation, textile and paper. Amylase is a hydrolytic enzyme and in recent years, interest in its microbial production has increased dramatically due to its wide spread use in food, textile, baking and detergent industr...

The alpha-amylases in the salivary glands of Lygus hesperus Knight and L. lineolaris (Palisot de Beauvois) were isolated and purified by ion exchange chromatography, and by isoelectric focusing, respectively. The alpha-amylase from L. hesperus had an isoelectric point (pI) of 6.25, and a pH optimum of 6.5. The specific activity of alpha-amylases in the salivary glands of L. hesperus was 1.2 U/m...