24 A rapid two step DNA extraction method and a multiplex PCR for the detection of 25 dermatophytes in general and Trichophyton (T.) rubrum specifically were developed and 26 evaluated on DNA extracted from pure cultures and from clinically diseased nails. DNA 27 from the following dermatophytes were used: Epidermophyton floccosum, Microsporum 28 (M.) audouinii, M. canis, M. gypseum, M. nanum, ...

The main aim of this research is to formulate and evaluate polyherbal anti-dandruff shampoo against the Malassezia furfur, by using ingredients from natural sources. herbs were extracted simple decoction process. Most effective herbs-Shikakai, Reetha, Neem, Aloe vera, Hibiscus, Fenugreek, Onion juice, Sidar powder, Curry leaves chosen in order improve activity. formulations evaluated for physic...

As part of research exploring the feasibility of using antibody fragments to inhibit the growth of organisms implicated in dandruff, we isolated antibody fragments that bind to a cell surface protein of Malassezia furfur in the presence of shampoo. We found that phage display of llama single-domain antibody fragments (VHHs) can be extended to very harsh conditions, such as the presence of shamp...

background: malassezia furfur is alipophilic yeast that causes skin disease. objective: to evaluate the level of il-10, ifn-γ and il-12p70 in co-incubation of m. furfur grown on different forms of natural oils with pbmcs of healthy individuals. methods: pbmcs were obtained from blood samples of normal volunteers. m. furfur was cultured in different culture media containing almond oil, fish oil,...

Phylogenetic relationships among heterobasidiomycetous yeasts, including anamorphic and teleomorphic taxa, have been compared from the sequence similarity of small (18S) and large (25S) subunit ribosomal RNA. Species examined were Cystofilobasidium capitatum, Filobasidiella neoformans, Fi/obasidium floriforme, Leucosporidium scottii, Malassezia furfur, M. pachydermatis, Phaffia rhodozyma, Rhodo...

Melanin-like pigment produced in vitro and in vivo by Malassezia yeasts has not been described before. Masson-Fontana staining confirmed accumulation of black pigment on the cell walls of L-dihydroxyphenylalaline (L-DOPA)-cultured Malassezia species. Black pigment was also observed in cells and hyphae from hyperpigmented patient lesions with culture-confirmed pityriasis versicolor and seborrhei...

Psoriasis, a common cutaneous disease of unknown etiology, may be triggered by infections, including those due to fungi. Since the fungal community of human skin is poorly characterized, we aimed to analyze the mycological microbiota in healthy skin and psoriatic lesions. Twenty-five skin samples from five healthy subjects (flexor forearm) and three patients with psoriasis were analyzed using b...

Dermatophyte and yeast species have been screened for their degradative ability towards various keratin substrates. Application of soluble preparation of keratin (KS) of chicken feathers enables a preliminary evaluation of the growth of the fungi and screening of fungal isolates that possesed keratinolytic activity and keratinase enzyme. Five dermatophytes i.e. Trichophyton rubrum, Trichophyton...

BACKGROUND Malassezia yeasts are normal flora of the skin that are discovered in 75~98% of health subjects, but since its association with various skin disorders have been known, many studies have been conducted in the distribution of the yeasts. OBJECTIVE To isolate, identify, and classify Malassezia yeasts from the normal human skin of Koreans by using the rapid and accurate molecular biolo...