Diagnosis of asymptomatic malaria poses a great challenge to global disease elimination efforts. Healthcare infrastructure in rural settings cannot support existing state-of-the-art tools necessary to diagnose asymptomatic malaria infections. Instead, lateral flow immunoassays (LFAs) are widely used as a diagnostic tool in malaria endemic areas. While LFAs are simple and easy to use, they are u...

BACKGROUND Malaria is a major cause of morbidity and mortality. Treatment of malaria in a timely manner could avert deaths. Treatment ultimately relies on the rapid and accurate diagnosis. Fluorescence in situ hybridization (FISH), a cytogenetic technique based on detection of specific nucleic acid, has the potential to address the limitations of the current diagnostic approaches. This study in...

The present study was conducted to find out the prevalence of malaria in human population of selected four villages of district Okara, province Punjab, Pakistan. Malaria is endemic from the last few years in this area. Blood samples were collected by passive case detection method from four private clinics and a major Basic Health Unit (BHU). Prevalence was determined using slide positivity rate...

GOALS Automated flowcytometric analysers are used to analyse complete blood count (CBC) in most patients. They differentiate WBC depending on their size, internal granularity, and nuclear content by producing various scattergrams. In malaria, haemozoin pigment-containing cells (parasitized RBC and phagocytic cells) are able to depolarize the laser beam and therefore exhibit abnormal scattergram...

A fast, precise, noninvasive, high-throughput, and simple approach for detecting malaria in humans and mosquitoes is not possible with current techniques that depend on blood sampling, reagents, facilities, tedious procedures, and trained personnel. We designed a device for rapid (20-second) noninvasive diagnosis of Plasmodium falciparum infection in a malaria patient without drawing blood or u...

Leishmaniasis is a disease caused by a malaria-like parasite called Leishmania in human and some species of animals. Detection of leishmaniasis has always been crucial for control and treatment of the disease. Different strategies have been approached for detection of leishmania. In this review methods used for detection of leishmania infection have been discussed and compared. Key word: Leishm...

R apid and accurate diagnosis is a key to the success of an effective treatment of malaria. The global impact of malaria has spurred interest in developing diagnostic strategies that will be effective not only in resource-limited areas but also in developed countries, where expertise in malaria diagnosis is often lacking. Accurate diagnosis of malaria is necessary to prevent morbidity and morta...