The paper deals with a stochastic multi-product sequencing and lot-sizing problem for a line that produces items in lots. Two types of uncertainties are considered: random lead time induced by machine breakdowns and random yield to take into account part rejects. In addition, sequence dependent setup times are also included. This study focuses on maximizing the probability of producing a requir...

The paper deals with a stochastic multi-product sequencing and lot-sizing problem for a line that produces items in lots. Two types of uncertainties are considered: random lead time induced by machine breakdowns and random yield to take into account part rejects. In addition, sequence dependent setup times are also included. This study focuses on maximizing the probability of producing a requir...

DNA sequencing using the fluoresence based Sanger method comprises interpretation of a sequence of signal peaks of varying size whose colour indicates the presence of a base. We have established that the ability to predict the variations effectively makes available novel error correction information which will improve sequencing efficacy. Our experiments have used basic models of the Sanger rea...

MOTIVATION Base-calling of sequencing data produced by high-throughput sequencing platforms is a fundamental process in current bioinformatics analysis. However, existing third-party probabilistic or machine-learning methods that significantly improve the accuracy of base-calls on these platforms are impractical for production use due to their computational inefficiency. RESULTS We directly f...

BACKGROUND Treatment for patients with advanced non-small cell lung cancer (NSCLC) is often determined by the presence of biomarkers that predict the response to agents targeting specific molecular pathways. Demands for multiplex analysis of the genes involved in the pathogenesis of NSCLC are increasing. METHODS We validated the Ion Torrent Personal Genome Machine (PGM) system using the Ion A...

The two strongest factors predicting a human cancer’s clinical behaviour are the primary tumour’s anatomic organ of origin and its histopathology. However, roughly 3% of the time a cancer presents with metastatic disease and no primary can be determined even after a thorough radiological survey. A related dilemma arises when a radiologically defined mass is sampled by cytology yielding cancerou...

Identification of single nucleotide polymorphisms (SNPs) is a key element in sequence-based genetic analysis. Next generation sequencing offers a cost-effective basis to generate the necessary, large sequence data sets, and bioinformatic methods are being developed to process sequencing machine readouts. We were interested in detection of SNPs in a 350 kb region of an EMS-mutagenized Arabidopsi...