Estimations of RNA abundance in solid tissues by nuclease protection assays (1, 2) are difficult, because of problems attaining efficient RNA extraction and because of RNA instability during molecular hybridization or after denaturation of nuclease-treated, probe:target hybrids. A method to overcome these difficulties in the case of tissue cultured cells was published (3) which conducted molecu...

MOTIVATION Proteins play a crucial role in biological activity, so much can be learned from measuring protein expression and post-translational modification quantitatively. The reverse-phase protein lysate arrays allow us to quantify the relative expression levels of a protein in many different cellular samples simultaneously. Existing approaches to quantify protein arrays use parametric respon...

Mice which had been subjected to mycobacterial infection and specific elicitation were bled from the orbital sinus at the time the mice were challenged intravenously with vaccinia virus. Assays of the sera were negative for interferon. Sera of rabbits sensitized with staphylococci and elicited by Staphage Lysate also lacked detectable circulating interferon.

Endpoint determinations of endotoxin by the Limulus amoebocyte lysate assay have been facilitated by use of a microdilution procedure.

Semisynthetic penicillins in concentrations greater than 1.0% (wt/vol) were found to inhibit the limulus lysate assay for endotoxin.

It has been reported that plasma endotoxin, measured by Limulus amoebocyte lysate assays, markedly increased during extracorporeal circulation (ECC). Therefore, this study was undertaken to see if pretreatment with methylprednisolone 30 mg kg-1 modifies the endotoxaemia and associated increases in plasma cytokines in 17 patients undergoing cardiac surgery requiring ECC. We found that methylpred...

N. Leigh Anderson Molecular Anatomy Program Division of Biological and Medical Research Argonne National Laboratory Argonne, Illinois 60439 Received June 6,1979 SUMMARY: Using the technique of thermal denaturation in a temperature gradient followed by two-dimensional gel electrophoresis of the fractions, the thermostabilities of the cytoplasmic actins have been investigated. Addition of 5 mM Mg...

METHODS. Recombinant human C-crystallin was mixed with various concentrations of glutathione (GSH) and diamide at 25°C for 1 hour. The extent of glutathiolation of the Ccrystallin was determined by mass spectrometry. Native and S-glutathiolated C-crystallins were labeled with I, and proteolytic degradation was determined using both lens fiber lysate and reticulocyte lysate as sources of ubiquit...

Twenty-eight antibiotics were tested with the Limulus amoebocyte lysate assay to determine their non-inhibitory concentrations (NICs). The Limulus amoebocyte lysate assay was found to be a valid test for most of the antibiotics tested; the NICs were found to be greater than the minimum valid test concentrations. Borderline results were obtained with cefamandole nafate and neomycin sulfate. Poly...