Density-labeling with 10 millimolar K(15)NO(3)/70% (2)H(2)O has been used to investigate isocitrate lyase synthesis during greening of sunflower (Helianthus annuus L.) cotyledons when the glyoxysomal enzyme activities sharply decline and the transition in cotyledonary microbody function occurs. A density shift of 0.0054 (kilograms per liter) was obtained for the profile of isocitrate lyase acti...

1. Malyl-CoA lyase was found in high activity in extracts of Pseudomonas AM1, Pseudomonas MA, Pseudomonas MS, Hyphomicrobium X and Methylosinus trichosporium. 2. The enzyme cleaves (2S)-malyl-CoA into equimolar amounts of acetyl-CoA and glyoxylate in the presence of Mg(2+). 3. The specific activity of malyl-CoA lyase was several-fold higher in Pseudomonas AM1 when grown on C(1) compounds than w...

Oleuropein, Hydroxytyrosol and Tyrosol are three major phenolic compounds present in olive with remarkable pharmacological effects. In this study, leaves and fruit of four olive cultivars including ‘Fishomi’, ‘Mary’, ‘Roghani’ and ‘Zard’ grown in the north of Iran were collected for comparative evaluation of the concentration of Oleuropein, Hydroxytyrosol and Tyrosol as well as antioxidant prop...

The sequence of a phenylalanine ammonia-lyase (PAL; EC: 4.3.1.24) of the thermophilic and radiotolerant bacterium Rubrobacter xylanophilus (RxPAL) was identified by screening the genomes of bacteria for members of the phenylalanine ammonia-lyase family. A synthetic gene encoding the RxPAL protein was cloned and overexpressed in Escherichia coli TOP 10 in a soluble form with an N-terminal His6-t...

The transcript levels of six defense genes including pathogenesis-related gene 1 (PR-1), pathogenesis-related gene 2 (PR-2), pathogenesis-related gene 5 (PR-5), lipoxygenase (LOX), phenylalanine ammonia-lyase (PAL) and catalase (CAT) were investigated in tomato plants inoculated with Xanthomonas axonopodis pv. phaseoli as a non-host pathogen and X. euvesicatoria as a host pathogen. Activation o...

The aim of this study was to evaluate the physicochemical parameters mangoes for export. results revealed that lose firmness after three days storage and pulp turns yellow-orange. sugar content increases progressively along time. However, phenylalanine ammonia-lyase (PAL) activity decreases with time confirming decrease phenol content. As polyphenoloxidases (PPO) peroxidases (POD), it increased...

Using DNA probes specific for the three members of the bean (Phaseolus vulgaris L.) phenylalanine ammonia-lyase (PAL) gene family, we have studied the effects of the product of the PAL reaction, trans-cinnamic acid (CA), on the appearance of individual PAL transcripts in suspension cultured bean cells. Concentrations of CA in excess of 10(-4) molar inhibited appearance of elicitor-induced trans...

A randomized complete block design was used to characterize the relationship between production of total phenolics, flavonoids, ascorbic acid, carbohydrate content, leaf gas exchange, phenylalanine ammonia-lyase (PAL), soluble protein, invertase and antioxidant enzyme activities (ascorbate peroxidase (APX), catalase (CAT) and superoxide dismutase (SOD) in Labisia pumila Benth var. alata under f...

Xanthan-modifying enzymes are powerful tools in studying structure-function relationships of this polysaccharide. One of these modifying enzymes is xanthan lyase, which removes the terminal side chain residue of xanthan. In this paper, the cloning and sequencing of the first xanthan lyase-encoding gene is described, i. e., the xalA gene, encoding pyruvated mannose-specific xanthan lyase of Paen...

The color of grapevine berries is one the most important quality parameters that define appearance, attracts consumer, and determines price, especially in red grape cultivars. Some varieties show less development due to many factors such as temperature, light intensity, agricultural management. present study aimed evaluate diverse phytohormones coupled with grafting on fruit coloration ’Crimson...