In contrast to conventional static cold preservation (0-4 °C), ex situ machine perfusion may provide better preservation of donor livers. Continuous perfusion of organs provides the opportunity to improve organ quality and allows ex situ viability assessment of donor livers prior to transplantation. This video article provides a step by step protocol for ex situ normothermic machine perfusion (...

A procedure for the perfusion of the isolated chicken liver was developed. The preparation satisfied many of the criteria of normal physiological function, e.g. oxygen consumption and the absence in the perfusion medium ofenzymes indicative of cell damage, and retained its capacity to synthesize lipids from glucose, acetate and long-chain fatty acids. Part ofnewly synthesized triglyceride was r...