The concentrations of the inner mitochondrial membrane markers cardiolipin and cytochrome alpha have been measured in liver homogenates and in purified mitochondria after thyroxine administration to thyroidectomized and normal rats. The biochemical results have been correlated with stereological electron micrographic analyses of hepatocytes in liver sections, and of isolated mitochondrial pelle...

The relationship between the expression of mitochondrial voltage-dependent anion channels (VDACs) and the protective effects of Myrica rubra Sieb. Et Zucc fruit extract (MCE) against carbon tetrachloride (CCl(4))-induced liver damage was investigated. Pretreatment with 50 mg kg(-1), 150 mg kg(-1) or 450 mg kg(-1) MCE significantly blocked the CCl(4)-induced increase in both serum aspartate amin...

OBJECTIVE To investigate the abundance of metabolic proteins in adult and fetal human livers. METHODS Adult liver homogenate proteins, fetal liver homogenate proteins, adult liver mitochondrial proteins and fetal liver mitochondrial proteins were obtained from fetal or adult liver tissues and examined using the antibody microarrays containing 19 liver monoclonal mitochondrial antibodies. The ...

Abstract Background To identify the role of physical prehabilitation (PP) in liver regeneration, mitochondrial function, biogenesis, and inflammatory response was investigated after associating partition portal vein ligation for staged hepatectomy (ALPPS) a rodent model. Methods Male Wistar rats (n = 60) underwent ALPPS. Animals were divided 30) to group sedentary (S). The animals exsanguinated...

BACKGROUND Cardiolipin (CL) helps maintain mitochondrial structure and function. Here we investigated whether a high carbohydrate diet (HCD) fed to mice for short period (5 days) could modulate the CL level, including that of monolysoCL (MLCL) in liver. RESULTS Total HCD group was 22% lower than normal chow (NCD) (P < 0.05). The CL72:8 level strikingly decreased by 93% (P < 0.0001), whereas tot...

C. Zwingmann, C. Éthier, D. Leibfritz, M. Bilodeau Centre de Recherche, Laboratoire d'hepatologie cellulaire, Hospital Saint-Luc (CHUM), Montreal, Quebec, Canada, Department of Organic Chemistry, University of Bremen, Bremen, Germany Introduction Mitochondrial damage is being recognized as a key step in liver injury associated with oxidative stress. Mitochondria also have necessary roles in the...

No-flow ischemia occurs during cardiac arrest, hemorrhagic shock, liver resection and transplantation. Recovery of blood flow and normal physiological pH, however, irreversibly injures the liver and other tissues. Although the liver has the powerful machinery for mitochondrial quality control, a process called mitophagy, mitochondrial dysfunction and subsequent cell death occur after reperfusio...

To examine the effect of 50% food restriction over a period of 3 days on mitochondrial energy metabolism, liver mitochondria were isolated from ad libitum and food-restricted rats. Mitochondrial enzyme activities and oxygen consumption were assessed spectrophotometrically and polarographically. With regard to body weight loss (-5%), food restriction decreased the liver to body mass ratio by 7%....

Introduction: Recently depleted uranium is being widely used as anti-armour ammunition and at very high temperature, results in information of an aerosol of very small uranium oxide particles, which may be inhaled. It is alleged that these particles represent a new battlefield hazard because of the chemical toxicology and/or radioactivity. Method & materials: Male Wistar strain albino rats ...

Although the biokinetics, metabolism, and chemical toxicity of methyl tert-butyl ether are well known, little attention was paid to the potential toxic effects of MTBE on reproduction and development in mammals. To evaluate the effects of MTBE on pregnant animals, two groups (control and test) of NMRI mice were chosen. In test group 500 and 1000 mg/Kg of it were administered intraperitonealy at...