Background: Gp96 is the cellular receptor for Vip, a Listeria monocytogenes surface protein. The molecular details of Gp96-Vip interaction are unknown. Results: Cell surface expression of Gp96 increases upon Listeria infection. Gp96 Nterminal is required for Vip interaction. Conclusion: Gp96 N-terminal domain is exposed to the extracellular milieu and is required for optimal Listeria entry. Sig...

In September 2015, PulseNet, the national molecular subtyping network for foodborne disease surveillance, identified a cluster of Listeria monocytogenes (Listeria) clinical isolates indistinguishable by two-enzyme pulsed-field gel electrophoresis (PFGE) pattern combination and highly related by whole-genome multilocus sequence typing (wgMLST). A case was defined as isolation of Listeria with th...

A molecular method based on restriction fragment length polymorphism (RFLP) of PCR-amplified fragments of the 23S rRNA gene was designed to rapidly identify Listeria strains to the species level. Two fragments (S1, 460 bp, and S2, 890 bp) were amplified from boiled DNA. S2 was cut with the restriction enzymes XmnI or CfoI and, if needed, S1 was digested by either AluI or ClaI. This method was f...

Food contaminated with human pathogens, such as Salmonella spp. and Listeria monocytogenes, frequently causes outbreaks of foodborne illness. Consumer concern over the use of synthesized antimicrobials to enhance microbial food safety has led to a search of natural alternatives. The objectives of this study were to evaluate the antimicrobial activity of various types of sophorolipids (SLs) and ...

A total of 154 samples of marine (n=51) and freshwater fish (n=103) were obtained from fish markets in Elazig Province of eastern Turkey. These samples were tested for Campylobacter, Listeria and Salmonella using culturing and biochemical methods. Campylobacter failed to be detected in any freshwater or marine fish samples. Listeria was detected in 22 and 14 of gill and skin samples from freshw...

Introduction: Listeria monocytogenes is a pathogen bacteria transmitted by food stuffs. Due to the lack of information about contamination of retail chicken meat to Listeria monocytogenes in Iran, this study aimed to determine the prevalence and antibiotic resistance of this bacterium in retail chicken meat in Yazd, Iran. Materials and Methods: This cross-sectional descriptive study was conduc...

A method for the rapid lysis of Listeria cells, employing a recombinant Listeria bacteriophage A118 lytic enzyme (PLY118), is described. The procedure can be used with all listerial species. It enables fast, efficient, and gentle recovery of DNA, RNA, or native cellular proteins from small-scale (2- to 5-ml) cultures. Moreover, this approach should be very useful in analytical detection and dif...

Spleen cells of mice infected with Listeria monocytogenes were adoptively transferred to normal mice. Such lymphocytes conferred resistance to a lethal challenge with Listeria. Hyperimmunization of the donor reduces the number of cells necessary to transfer effective immunity. Such spleen cells if treated with anti-theta serum do not transfer resistance to Listeria. Hence, thymus (T) lymphocyte...

Background Listeria sp. are facultative intracellular bacterial pathogens, which pose a potential public health problem related to consumption of contaminated food. Human listeriosis is clinically classified as perinatal listeriosis, neonatal listeriosis and adult listeriosis. Human infections caused by Listeria sp. present typically as meningitis. Other infections attributed to Listeria sp. in...

Background: Listeria consists of both pathogenic and non-pathogenic species. Reports of similarities between the genomic content between some pathogenic and non-pathogenic species necessitates the investigation of these species at the genomic level to understand the evolution of virulence-associated genes. With Listeria genome data growing exponentially, comparative genomic analysis may give be...