INTRODUCTION Glucocorticoids are commonly used as therapeutic agents in many acute and chronic inflammatory and auto-immune diseases. The current study investigated the effects of methylprednisolone (a synthetic glucocorticoid) on aortic distensibility and vascular resistance in lipopolysaccharide-induced chronic inflammation in male Wistar rats. METHODS Chronic inflammation was induced by im...

signaling amp-activated protein kinase (ampk), an energy sensing enzyme, has been implicated in controlling inflammation. in this study we investigated whether activation of ampk by metformin could protect the lung from lipopolysaccharide (lps)-induced acute injury by inhibitingng tlr4 pathway. male wistar rats were randomly divided into 3 groups (n=6): control group received normal saline (0.5...

background and objective: escherichia coli o157:h7, an emerging pathogen, causes severe enteritis and the extraintestinal complication of hemolytic-uremic syndrome. the goal of this study was to evaluate the conjugate of e. coli o157: h7 lipopolysaccharide (lps) with diphtheria toxoid (dt) as a candidate vaccine in mice model. material and methods: lps from e. coli o157:h7 was extracted by hot ...

A Pasteurella haemolytica A1 gene involved in the biosynthesis of a moiety on the core of the lipopolysaccharide molecule has been cloned and characterized. Escherichia coli clones which carry this gene showed an alteration of its lipopolysaccharide migration profile on tricine SDS-PAGE and exhibited resistance to the core-specific phage U3. In addition, lipopolysaccharide extracted from the E....

Serum samples from 22 patients with proven typhoid fever, 60 febrile nontyphoidal patients, and 120 healthy subjects were tested for immunoglobulin A (IgA), IgG, and IgM anti-Salmonella typhi lipopolysaccharide antibodies by an enzyme-linked immunosorbent assay. The levels of all three classes of immunoglobulin anti-lipopolysaccharide were higher in typhoid patients than in controls; the test f...

BACKGROUND Acute lung injury is characterized by neutrophilic inflammation and increased lung permeability. Thiosulfate is a stable metabolite of hydrogen sulfide, a gaseous mediator that exerts antiinflammatory effects. Although sodium thiosulfate (STS) has been used as an antidote, the effect of STS on acute lung injury is unknown. The authors assessed the effects of STS on mice lung and vasc...

OBJECTIVES Animals administered lipopolysaccharide exhibit dose-related sickness behaviors (decreased food intake, weight loss, and cognitive changes). While research has demonstrated that spatial learning is impaired following a lipopolysaccharide immune challenge, the results differ depending on the methodology used to evaluate spatial learning. Additionally, few studies have evaluated the ef...

RATIONALE Endothelial injury is an initial mechanism mediating cardiovascular disease. OBJECTIVE Here, we investigated the effect of hyperhomocysteinemia on programed cell death in endothelial cells (EC). METHODS AND RESULTS We established a novel flow-cytometric gating method to define pyrotosis (Annexin V(-)/Propidium iodide(+)). In cultured human EC, we found that: (1) homocysteine and l...

OBJECTIVE We hypothesized that fetal innate immune responses to lipopolysaccharide-induced chorioamnionitis would alter postnatal systemic immune and airway responsiveness. STUDY DESIGN Ewes received intraamniotic injections with saline or lipopolysaccharide at 90, 100, and 110 days of gestation. Immune status and airway responsiveness were evaluated at term and at 7 weeks of age. RESULTS A...

The dissociation of the phospholipid, lipopolysaccharide, and protein components of the bacterial cell envelope results in loss of enzyme activities which are normally located in the cell envelope structure and which are involved in lipopolysaccharide biosynthesis. The activity of one of these enzymes, UDP-galactose:lipopolysaccharide alpha,3 galactosyl transferase, can be restored by the reass...