نتایج جستجو برای: linked immunosorbent assay in host range studies

تعداد نتایج: 17238930  

Journal: :Journal of clinical microbiology 1980
J D Shanley

An enzyme-linked immunosorbent assay for immunoglobulin G antibody to encephalomyocarditis virus was developed. This assay was comparable to antibody assay by neutralization. Its adaptability should be useful for laboratory and epidemiological studies of infections due to encephalomyocarditis virus.

Journal: :Journal De Mycologie Medicale 2021

Pythium insidiosum causes a life-threatening infection termed pythiosis in humans and other animals. The organism has been identified tropical subtropical environments worldwide. Since 1985, human increasingly reported from Thailand. Seroprevalence studies estimated that 32,000 Thai people had exposed to the pathogen. In 2018, first animal case Thailand was diagnosed horse. Here, we investigate...

2012
Katherine M. Smith Kavita Nanda Victoria McCarl Carla J. Spears Amanda Piper Mariana Ribeiro Michelle Quiles Caitlin M. Briggs Gwynneth S. Thomas Malcolm E. Thomas Dennis T. Brown Raquel Hernandez

The immunogenicity and safety of three novel host-range vaccines containing deletions in the transmembrane domain of dengue virus serotype 2 (DV2) E glycoprotein were evaluated in African green monkeys. The shorter transmembrane domains are capable of functionally spanning an insect but not a mammalian cell membrane, resulting in production of viral mutants that have reduced infectivity in mamm...

Hasan Marashi Maziar Habibi-Pirkoohi, Mohammadreza Nassiri, Nasrin Moshtaghi, Saeid Malekzadeh-Shafaroudi Saeid Zibaee

Background: Transient and stable transformation of host plants are the common techniques to produce transgenic plants. However, the main drawback of stable transformation is the fact that it takes quite a long time to produce a transgenic line. While, transient gene expression is a quick method to produce recombinant proteins in plants.  Objective: The main goal of the present study was to eva...

Journal: :Clinical and vaccine immunology : CVI 2006
Nidia M M Oliveira Annette K Broom John S Mackenzie David W Smith Michael D A Lindsay Brian H Kay Roy A Hall

We describe the development of an epitope-blocking enzyme-linked immunosorbent assay (ELISA) for the sensitive and rapid detection of antibodies to Ross River virus (RRV) in human sera and known vertebrate host species. This ELISA provides an alternative method for the serodiagnosis of RRV infections.

Journal: :Journal of clinical microbiology 1988
N Schmeer H P Müller W Baumgärtner J Wieda H Krauss

A microtiter enzyme-linked immunosorbent fluorescence assay based on alkaline phosphatase conjugate and 4-methylumbelliferyl phosphate as fluorogenic substrate was developed and adapted to quantitatively analyze immunoglobulin G subclass 1 (IgG1) and IgG2 responses of vaccinated and infected cattle to proteins of Coxiella burnetii. The enzyme-linked immunosorbent fluorescence assay surpassed th...

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