We present a device and method for selective chemical interactions with immersed substrates at the centimeter-scale. Our implementations enable both, sequential and simultaneous delivery of multiple reagents to a substrate, as well as the creation of gradients of reagents on surfaces. The method is based on localizing submicroliter volumes of liquids on an immersed surface with a microfluidic p...

conclusions the in-house 1 step taqman real time rt-pcr assay showed acceptable performance characteristics. our study presents the robustness and cost-effectiveness of the method for detection and quantification of hcv rna. methods the primers and probe were selected from a highly conserved region of the hcv genome, which allowed the detection of 4 common hcv genotypes in iran. using 4 quantif...

A panel of melanoma cell lines derived from 7 primary and 20 metastatic lesions was tested for the production of interleukin 1 (IL-1) in standard mouse thymocyte costimulation assays. Constitutively produced IL-1 activity was found in the conditioned media of 4 of 7 primary and 5 of 20 metastatic melanoma cell lines tested. Four of 9 cell lines secreting IL-1 were also shown to contain cell-ass...

A method of construction of A-optimal binary block designs for multiple asymmetrical parallel line assays has been proposed. Illustration of the method with examples has been provided. By this method two series of designs are obtained. The first series of designs have equal replication of treatments with unequal block sizes. The second series of designs have equal block sizes with unequal repli...