Banana bunchy top virus (BBTV) affects all varieties of banana plants and causes heavy economic loss in most of the banana cultivating areas. The BBTV genome comprises of six DNA components; in this study, we have cloned the six BBTV-DNA components from one of the BBTV-infected plants (Tri-8) and were submitted to GenBank. Analysis of the BBTV DNA-R component showed that it belonged to south Pa...

sayed by liquid chromatography, immunoassay, or capillary electrophoresis. The separation systems offer selective detection of analytes, but in many cases, the temporal resolution of the assay is not sufficient for continuous real-time monitoring of the microdialysate. These techniques are limited by the minimum sample volume required for practical sample handling and reliable measurements. The...

Abstract RNA-based therapies have great potential to treat many undruggable human diseases. However, their efficacy, in particular for mRNA, remains hampered by poor cellular delivery and limited endosomal escape. Development optimisation of vectors, such as lipid nanoparticles (LNPs), are impeded screening methods probe the intracellular processing LNPs sufficient detail. We developed a high-t...

The control of the three-dimensional (3D) polymer network structure is important for permselective materials when specific biomolecule detection needed. Here we investigate conditions to obtain a tailored hydrogel that combines both molecular filtering and capture capabilities biosensing applications. Along this line, short oligonucleotide in displacement assay set within PEGDA hydrogels synthe...

We conducted a comparative evaluation of the Chlamydiazyme (Abbott Laboratories), PACE 2 (Gen-Probe), and AMP-CT (Gen-Probe) assays for the detection of Chlamydia trachomatis in endocervical samples. Specimens from 787 females were included in the study. The sensitivities of the PACE 2 and Chlamydiazyme assays in comparison to the results of the AMP-CT assay were 79.3 and 63.4%, respectively. T...

Culture is currently considered the "gold standard" for detecting Chlamydia trachomatis infections. We evaluated the Syva MicroTrak enzyme immunoassay (EIA) and Gen-Probe PACE 2 tests, which detect chlamydial antigens and rRNA, respectively. These assays were compared with each other and with culture for the detection of C. trachomatis in cervical specimens obtained from 217 women attending a c...

The Gen-Probe PACE 2 assay (Gen-Probe Inc., San Diego, Calif.) was compared with culture for the detection of Neisseria gonorrhoeae in endocervical specimens that were mailed to the laboratory. During mail transport, the specimens were exposed to extremes of hot and cold weather for several days before arriving in the laboratory. Specimens on culture plates deteriorated during transport, as evi...