background: limiting dilution assay is considered as the gold standard method for quantifying the number of parasites in the animal model of leishmania infection. nowadays, real-time pcr is being increasingly applied to quantify infectious agents. in the present study , a real-time pcr assay was developed to estimate para­site burdens in lymph nodes of leishmania major infected balb/c mice. enu...

conclusions the results of this study illustrated the persistence of resistant bacteria in the environment, and highlight the reservoir of resistance, associated with use of antibiotics, as feed additive in poultry production. results out of the 55 mrsa strains, all isolates were at least resistant to penicillin, 58% showed resistance to erythromycin and 55% were resistant to ciprofloxacin. on ...