An artificial retroaldolase was developed using a 43-amino-acid-long miniprotein derived from the C-terminal domain of MvaT protein. A series rounds rational optimization activity performed, included grafting active motif in cleft miniprotein, engineering site structure, and modifying charge distribution on surface miniprotein. It proved that miniproteins this size can be mutated to obtain an e...

La púrpura trombótica trombocitopénica (PTT) es una microangiopatía poco frecuente, que se caracteriza por anemia hemolítica y plaquetopenia, con elevada morbimortalidad. Su forma más frecuente la PTT inmune, también denominada adquirida, provocada deficiencia de enzima disintegrin-like and metalloprotease with thrombospondin type 1 motif 13 (ADAMTS13) secundaria a presencia en plasma autoantic...

We present a novel classification-based algorithm called GeneClass for learning to predict gene regulatory response. Our approach is motivated by the hypothesis that in simple organisms such as Saccharomyces cerevisiae, we can learn a decision rule for predicting whether a gene is upor down-regulated in a particular experiment based on (1) the presence of binding site subsequences (“motifs”) in...

In vertebrates, the conversion of fibrinogen into fibrin is an essential process that underlies the establishment of the supporting protein framework required for coagulation. In invertebrates, fibrinogen-domain-containing proteins play a role in the defense response generated against pathogens; however, they do not function in coagulation, suggesting that this role has been recently acquired. ...

Helquat dyes are the first helicene-like cationic styryl dyes obtained as separate enantiomers. Their remarkable chiroptical properties are due to the unique combination of a cationic hemicyanine chromophore and a helicene-like motif. The magnitude of the ECD response and the pH switching along with their positioning in the visible region are unprecedented among helicenoids.

Comparative transfection analysis of murine interferon A4 and interferon A11 promoter constructs transiently transfected in mouse L929 and human HeLa S3 cells infected with Newcastle disease virus showed that the second positive regulatory domain I-like domain (D motif), located between nucleotides -57 and -46 upstream of the transcription start site, contributes to the activation of virus-indu...