CD341 hematopoietic stem cells from normal individuals and from patients with chronic myelogenous leukemia can be induced to differentiate into dendritic cells (DC). The aim of the current study was to determine whether acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL) cells could be induced to differentiate into DC. CD341 AML-M2 cells with chromosome 7 monosomy were cultured ...

PURPOSE To determine whether mTORC2 and rapamycin-insensitive (RI)-mTORC1 complexes are present in acute myeloid leukemia (AML) cells and to examine the effects of dual mTORC2/mTORC1 inhibition on primitive AML leukemic progenitors. EXPERIMENTAL DESIGN Combinations of different experimental approaches were used, including immunoblotting to detect phosphorylated/activated forms of elements of ...

5-Aza-2'-deoxycytidine administered at a daily dose of 1.5 mg/kg increased the life-span of P388 leukemia-bearing BALB/c X DBA/2 F1 mice by 5 times and that of second generation lymphoma-bearing AKR mice by 2.5 times. Higher doses (total dose, 20 mg/kg) led to favorable results when administered in two portions on Days 4 and 5 after the s.c. inoculation of leukemic cells. The same total dose gi...

By ROBERT SCHREK, STANLEY L. LEIThOLD, IRVING A. FRIEDMAN AND WILLIAM R. BEST A PREVIOUS PAPER described a slide-chamber method for the measurement of the radiosensitivity of lymphocytes from leukemic patients.1 The method was used to study 31 patients with chronic lymphocytic leukemia or with lymphosarcoma in leukemic phase.2 The patients showed considerable variability with respect to the rad...

Light microscope studies of organs and tissues of 10to 12month-old leukemic NZB/BL strain mice revealed changes characteristic of lymphoid leukemia and autoimmune disease. Electron microscope studies of ultrathin sections of lymph nodes, spleen, liver, bone marrow, thymus gland, kidneys, and lungs of the same leukemic mice demonstrated immature and mature murine leukemia virus particles of the ...

Chronic myeloid leukemia (CML) progresses through three distinct clinical stages: chronic phase, accelerated phase, and blast crisis. The progression to accelerated phase and blast crisis is driven by activation of oncogenes, inactivation of tumor suppressor genes, and/or amplification of the BCR-ABL fusion gene, which causes the chronic phase of the disease. The cell of origin of blast crisis ...

The ability of the tumor promotor 12-0-tetradecanoylphorbol 13-acetate (TPA) to induce differentiation of leukemic cells was studied in 10 cases of chronic lymphocytic leukemia (CLL). An increase in modal volume and an enhancement of the capacity of te leukemic cells to stimulate in mixed lymphocyte reaction (MLR) was seen in the majority of cases. A significant increase in Ia expression was ob...

To detect more precisely the minimal residual disease in acute lymphoblastic leukemia (ALL), two-color flow cytometric analysis for the detection of cell-surface antigen (CD10; CALLA) and nuclear terminal deoxynucleotidyl transferase (TdT) was performed in the six patients with CALLA-positive ALL coexpressing TdT. In all patients, the leukemic blasts coexpressed Ia (HLA-DR), CD9, CD19, CD20, CD...

By ROBERT SCHREK, STANLEY L. LEIThOLD, IRVING A. FRIEDMAN AND WILLIAM R. BEST A PREVIOUS PAPER described a slide-chamber method for the measurement of the radiosensitivity of lymphocytes from leukemic patients.1 The method was used to study 31 patients with chronic lymphocytic leukemia or with lymphosarcoma in leukemic phase.2 The patients showed considerable variability with respect to the rad...

Large granular lymphocyte (LGL) leukemia is characterized by the expansion of antigen-activated cytotoxic T lymphocytes. These leukemic cells are resistant to Fas-mediated apoptosis despite expressing high levels of Fas. We found that leukemic LGL from 19 patients displayed high levels of activated STAT3. Treatment of leukemic LGL with the JAK-selective tyrosine kinase inhibitor AG-490 induced ...