نتایج جستجو برای: ldha

تعداد نتایج: 345  

2013
Yu Matsuoka Kazuyuki Shimizu

Effect of the specific gene knockout on the main metabolism in Escherichia coli was reviewed, and the regulation mechanisms were clarified based on different levels of information such as gene expressions, enzyme activities, intracellular metabolite concentrations, and metabolic fluxes together with fermentation data. The effects of the knockout of such genes as pflA, pta, ppc, pykF, adhE, and ...

Journal: :The EMBO journal 2015
Yang Cao Wen-Ting Guo Shengya Tian Xiaoping He Xi-Wen Wang Xiaomeng Liu Kai-Li Gu Xiaoyu Ma De Huang Lan Hu Yongping Cai Huafeng Zhang Yangming Wang Ping Gao

Enhanced glycolysis is a main feature of pluripotent stem cells (PSCs) and is proposed to be important for the maintenance and induction of pluripotency. The molecular mechanism underlying enhanced glycolysis in PSCs is not clear. Using Dgcr8-/- mouse embryonic stem cells (ESCs) that lack mature miRNAs, we found that miR-290 cluster of miRNAs stimulates glycolysis by upregulating glycolytic enz...

2017
Yapeng Ji Chuanzhen Yang Zefang Tang Yongfeng Yang Yonglu Tian Hongwei Yao Xi Zhu Zemin Zhang Jiafu Ji Xiaofeng Zheng

This corrects the article DOI: 10.1038/ncomms15308.

2015
Xiao-Xing Wei Wei-Tao Zheng Xue Hou Jian Liang Zheng-Jun Li

The alcohol dehydrogenase promoter PadhE and mixed acid fermentation pathway deficient mutants of Escherichia coli were employed to produce poly(3-hydroxybutyrate) (P3HB) under microaerobic condition. The E. coli mutant with ackA-pta, poxB, ldhA, and adhE deletions accumulated 0.67 g/L P3HB, up to 78.84% of cell dry weight in tube cultivation. The deletion of pyruvate formate-lyase gene pflB dr...

2015
Zhen Chen He Sun Jinhai Huang Yao Wu Dehua Liu Guo-Qiang Chen

2-Butanone is an important commodity chemical of wide application in different areas. In this study, Klebsiella pneumoniae was engineered to directly produce 2-butanone from glucose by extending its native 2, 3-butanediol synthesis pathway. To identify the potential enzyme for the efficient conversion of 2, 3-butanediol to 2-butanone, we screened different glycerol dehydratases and diol dehydra...

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