PURPOSE To investigate systematically the early morphologic changes in axon terminals of adult mammalian rod and cone photoreceptors prepared as a sheet for subretinal transplantation. METHODS An in vitro system was designed to maintain adult porcine retinas for up to 48 hours. Photoreceptor sheets, prepared by vibratome sectioning, and full-thickness retinas were cultured at temperatures sim...

Acellular corneal stroma matrix has been used for corneal stroma engineering. However, because of its compact tissue structure, regrowth of keratocytes into the scaffold is difficult. Previously, we developed a sandwich model for cartilage engineering using acellular cartilage sheets. In the present study, we tested this model for corneal stroma regeneration using acellular porcine corneal stro...

Saccharides and temperature dual-responsive hydrogels have been prepared based on PNIPAAm copolymers containing phenylboronic acid (PBA) groups and used for harvesting cell sheets. The cell sheet could be released from the hydrogel layer at 37 °C simply by increasing sugar concentration, and could be more efficiently released at a lower temperature and elevated sugar concentration.