Bacterial recombineering typically relies on genomic incorporation of synthetic oligonucleotides as mediated by Escherichia coli λ phage recombinase β - an occurrence largely limited to enterobacterial strains. While a handful of similar recombinases have been documented, recombineering efficiencies usually fall short of expectations for practical use. In this work, we aimed to find an efficien...

inthis paper, the main aim is to introduce the class $mathcal{u}_p(lambda,alpha,beta,k_0)$ of $p$-harmonic mappings togetherwith its subclasses $mathcal{u}_p(lambda,alpha,beta,k_0)capmathcal {t}_p$ and $mathcal{u}_p(lambda,alpha,beta,k_0)capmathcal {t}_p^0$, andinvestigate the properties of the mappings in these classes. first,we give a sufficient condition for mappings to be in $mathcal{u}_p(l...

Genetic manipulation in enterohemorrhagic O157:H7 is currently E. coli restricted to recombineering, a method that utilizes the recombination system of bacteriophage lambda, to introduce gene replacements and base changes into the genome. Bacteriophage 933W is a prophage in inter alia E. coli O157:H7 strain EDL933, which encodes the genes ( ) for the production stx2AB of Shiga toxin which is th...

‎In this paper‎, ‎we study a class of boundary value problem‎ ‎involving the p-Laplacian oprator and singular nonlinearities‎. ‎We‎ ‎analyze the existence a critical parameter $lambda^{ast}$ such‎ ‎that the problem has least one solution for‎ ‎$lambdain(0,lambda^{ast})$ and no solution for‎ ‎$lambda>lambda^{ast}.$ We find lower bounds of critical‎ ‎parameter $lambda^{ast}$‎. ‎We use the method ...