the effect of different levels of osmolality and ph of lactose extender on the viability of bactrian camelspermatozoa was investigated. semen was collected from 10 bactrian camel bulls using modified bovineartificial vagina. in experiment i, the extenders consisted of 9, 10, 11, 12 and 13% lactose with respectiveosmolalities of 290, 333, 350, 376 and 419 mosm/kg and the ph of 6.9. in experiment...

The lacE and lacG genes from Streptococcus mutans have been isolated and characterized, and their nucleotide sequence has been determined. The lacE gene encodes the lactose-specific Enzyme II component of the phosphoenolpyruvate-dependent phosphotransferase system (PTS). The lacG gene encodes the phospho-beta-galactosidase which cleaves the lactose phosphate that is formed by the lactose PTS. T...

[14C]Lactose electroinjected into isolated rat hepatocytes is normally autophagocytosed, transferred to lysosomes and degraded by lysosomal beta-galactosidase, but at high concentrations of asparagine the transfer is inhibited and lactose accumulates in prelysosomal autophagic/endocytic vacuoles (amphisomes). The accumulation can be prevented by addition of yeast beta-galactosidase, which is tr...

OBJECTIVE To investigate the prevalence of lactose and fructose intolerance in children with chronic abdominal pain. METHODS Hydrogen breath tests were done to detect lactose and fructose malabsorption in 86 children with chronic abdominal pain (44 irritable bowel syndrome, 24 functional abdominal pain and 17 functional abdominal pain syndrome as per Rome III criteria) presenting to a Pediatr...

Oligosaccharides from regular milk and lactose-free were analyzed by Electrospray Ionization interface coupled with Ion Trap Mass Spectrometry (IT-MS). The negative mode mass spectrometry of sugar compositions was obtained either direct infusion Liquid Chromatography (LC). Hexose (Hex) the other oligomeric components observed in both milk. While lactose derivatives (deprotonated dimer, chloride...

pray-dried (SD) lactose was introduced to the pharmaceutical market in the 1960s as an excipient that enables direct compression of formulations in a simple manufacturing process (1). To this day, lactose remains one of the most popular excipients for active pharmaceutical ingredients (APIs) whose dose makes them suitable for direct compression. Several manufacturers make and sell SD lactose, a...

The only carbohydrate in milk is lactose, a disaccharide, which is formed of a molecule of glucose and a molecule of galactose. Cow's milk contains about 5 % lactose and human milk about 6'5-7 %. Lactose is hydrolysed to its monosaccharides principally by a specific enzyme, lactase, which is a f-galactosidase. There are also two other enzymes with ,3-galactosidase activitv, lysosomal acid 3-gal...

Lactose intolerance has been reviewed in published statements by the Protein Advisory Group of the United Nations System,’ the Food and Nutrition Board of the National Research Council,2 and the American Academy of Pediatrics, Committee on Nutrition.’ Each of these statements deals principally with the advisability of encouraging population groups with a high rate of lactose intolerance to cons...