A simple, rapid plasmid mini-prep procedure for lactococci and lactobacilli which gives high yields and can be performed on overnight broth cultures is presented. Large plasmids were isolated from both lactococci and lactobacilli, including a 70-kb plasmid from Lactobacillus acidophilus C7. The purity of the resulting plasmid DNA makes it suitable for subsequent molecular manipulations. The con...

conclusions as l. crispatus and l. jensenni were significantly higher in bv infected women and the cytotoxic effect of l. crispatus on tumoral cervical cells was high, introduction of new probiotics seems necessary. results l. crispatus, l. gasseri, l. iners, l. jensenii, l. acidophilus and l. rhamnosus were the most frequently occurring species in vagina of healthy iranian women. l. crispatus ...

introduction: irritable bowel syndrome (ibs) is a common gastrointestinal disorder in children that may lead to anxiety, frequent physician visits and school absenteeism. the aim of this study is to reviewe effects of probiotic for irritable bowel syndrome.   materials and methods: this study review articles about probiotic for irritable bowel syndrome in pubmed and google scholar.   results: m...

This review discusses opportunities and bottlenecks for cell factory development of Lactic Acid Bacteria (LAB), with an emphasis on lactobacilli and pediococci, their metabolism and genetic tools. In order to enable economically feasible bio-based production of chemicals and fuels in a biorefinery, the choice of product, substrate and production organism is important. Currently, the most freque...

Previous studies have shown that Self Arresting Caries Treatment (SACT) may be a useful technique to arrest active caries in Thai preschool children in remote areas. The objective of this study was to examine the effect of SACT on the levels of salivary mutans streptococci and lactobacilli in Thai children. Eighty-two subjects aged 4-6 years from primary school in a remote area of Thailand were...

BACKGROUND Biofilms on medical devices are a frequent reason for failure of the device. Voice prostheses in laryngectomized patients deteriorate within 3 to 4 months due to adhering biofilms, impeding proper functioning. Recently, we showed that these biofilms are dominated by Candida and lactobacilli. However, the early report of this finding lacked an in depth analysis of the species diversit...

A previous communication (2) indicated that heme-linked enzymes were not involved in the very considerable capacities of various representative microaerophilic lactobacilli for electron transport from a variety of oxidizable substrates to oxygen, but that specific flavin components were present in high concentration in each of these organisms. To determine the possible central role of flavin en...

The effect that vaginal lactobacilli have on trichomoniasis is not known. At 3 clinics for treatment of sexually transmitted disease, we recruited 521 female patients with trichomoniasis and 176 control subjects. All participants underwent physical examinations and testing for sexually transmitted infection and completed questionnaires. Low levels of vaginal lactobacilli were associated with tr...

Bacterial vaginosis (BV) is the most commonly treated female reproductive tract affliction, characterized by the displacement of healthy lactobacilli by an overgrowth of pathogenic bacteria. BV can contribute to pathogenic inflammation, preterm birth, and susceptibility to sexually transmitted infections. As the bacteria responsible for BV pathogenicity and their interactions with host immunity...