Five gentamicin assay procedures (a bioassay, an enzyme immunoassay, a latex agglutination inhibition test, a fluorescence immunoassay, and a radioimmunoassay) were evaluated to determine which was optimal for our laboratory. The evaluation was based on recovery and precision studies and results of analyses of patient samples, as well as technical assay performance factors. The latex agglutinat...

The purpose of this research was to collect efficacy data on biological, biochemical, and chemical fungicide seed treatments hemp (Cannabis sativa L.) mitigate damping-off enhance field stand establishment. Seed were evaluated in fields New York (NY), North Dakota (ND), Virginia (VA) at two planting dates each state 2020. A single lot a dual-purpose (fiber + grain) cultivar (‘Anka’) treated usi...

Background Bacillus thuringiensis (Bt) Cry toxins have specific toxicity to susceptible insects. They are being used in transgenic plants or spray to control insect pests in agriculture [1, 2, 3]. Cry3A toxins are used extensively for biological control of coleopteran larvae [4, 5]. A Bt886-Cry3Aa gene that exhibited a high activity against Coleoptera insects isolated Our laboratory. Insect bio...

Experiments were designed in a bioassay system to analyze the effect of elevated (from 5.9 mM to 7.5-45.9 mM) extracellular K+ concentration on the release of endothelium-derived relaxing factor. Segments of canine femoral artery with endothelium (donor segment) were mounted in an organ bath and perfused with modified Krebs-Ringer bicarbonate solution; the effluent from the donor segment was us...

Fall armyworm, Spodoptera frugiperda, is currently a major and invasive pest that threatening global maize production food security especially in sub–Saharan Africa. Its tolerance to several available synthetic pesticides has led research into novel management strategies. An vitro laboratory bioassay was conducted at Bindura University of Science Education determine the LD50 for AgriX365, botan...

Feed represents more than 70% of the total cost of producing live broilers. In typical broiler feeds, more than 60% of the total cost is used to provide energy. Furthermore, it is generally agreed that broilers eat to satisfy energy requirement, therefore broiler feeds are normally formulated to provide all nutrients according to diet energy content. Therefore, it is critically important to kno...

Field-collected 4th-instar Glyptotendipes paripes larvae from a natural lake in central Florida preferred small-sized (< 0.84-1.99-mm-diam) grains of sand to burrow in and make cases in the laboratory. The larvae buried or formed cases within 3 h of introduction into 120-ml paper cups containing 80 ml tap water and 20 g of the small-diameter sand. When placed in sand with grains of different si...

Factor VIII (F.VIII) and von Willebrand factor (VWF):Ag data collected by eight laboratories on a total of 336 obligatory carriers of hemophilia A and 137 normal women were used to answer several questions concerning the construction of linear discriminants for carrier detection. It was found: that a "universal" linear discriminant can be constructed which is suitable for use in all laboratorie...