essential oils, with plant origin, have been of special attention in cancer research during recent years. despite many reports on cytotoxic effects of plants from genus salvia, the potential application of their extracts in cancer therapy remains to be assessed in more precise and detailed examinations on the main cause of such effects. in this research, the cytotoxic effect and anticancer acti...

Nonexcitable cell types, fibroblasts of heart muscle or astrocytes, are well known for their spontaneous Ca(2+) oscillations. On the other hand, murine fibroblast (L929) cells are known to be deficient in cell-cell adhesive proteins and therefore lack gap junctions for cellular communication. However, these cells exhibit a unique property of collectively synchronized and spontaneous oscillation...

We investigated the mechanism of the pan-caspase inhibitor z-VAD-fmk's augmentation of TNFalpha-induced L929 cell death and found this mechanism differs from that of TNFalpha-induced L929 cell death. In the presence of 20 ng/ml TNFalpha, z-VAD-fmk initiated apoptosis and necrosis in the majority of L929 cells as measured by an agarose gel electrophoresis and lactate dehydrogenase(LDH)activity b...

objective: nucleostemin (ns) is a recently identified gene that is expressed mainly in the nucleoli of neuronal, embryonic and mesenchymal stem cells which plays a role in their self renewal. over expression of this gene has been reported in several cancer cell lines tumoral tissues including gastric, hepatic and prostate cancer. in this study, we investigated the effects of suppression of this...

background : the development of chemoresistance represents a major obstacle in the successful treatment of cancers such as non-hodgkin’s lymphoma (nhl). with the recognition of important roles for both p53 and its more recently described paralog p73 in mediating the activity of anti-cancer drugs, there has been increasing recognition that cellular resistance to anthracyclines could and does a...

Stemodia maritima L., is a shrub of the Plantaginaceae family, with some biological activities already described, such as: larvicide, antimicrobial, and anti-inflammatory. Thus, objective this work was to evaluate antioxidant, gastroprotective ethyl acetate extract from S. maritima. The phytochemical profile investigated through quantification total phenolic compounds, flavonoids, CCD analysis....

The aim of this study was to develop a porous chitosan scaffold with long-acting drug release as an artificial dressing to promote skin wound healing. The dressing was fabricated by pre-freezing at different temperatures (-20 and -80 °C) for different periods of time, followed by freeze-drying to form porous chitosan scaffolds with different pore sizes. The chitosan scaffolds were then used to ...

The role of the nitric oxide synthase (NOS) pathway in inhibiting the ability of Rickettsia prowazekii to initially infect (invade) mouse cytokine-treated, fibroblastic L929 cells and macrophagelike RAW264.7 cells and the ability of nitric oxide (NO) to damage isolated rickettsiae were investigated. Substantial amounts of nitrite (a degradation product of NO) were produced and the initial ricke...

We investigated optimal conditions for cytotoxicity to tumor cell lines by recombinant human tumor necrosis factor (rhTNF) and the effect of amino-terminal deletions on the bioactivity of the rhTNF molecule. Two of four deletion muteins (-4 and -7) of rhTNF exhibit 2- to 3-fold enhancement of cytotoxicity/cytostasis against a variety of human carcinomas, a fibrosarcoma, and a melanoma cell line...

Cytotoxicity is one of the most important problems in medical metal materials. Effect of different dental alloys on cytotoxic of L929 cells was evaluated by in vitro cytotoxicity-test (MTT method). Metal ion concentration was one of the factors leading to occurrence of cell toxicity. Thus metal ion concentration in cell culture fluid at different intervals of immersion periods were detected by ...