Glutamate receptors are implicated in the genesis of opioid tolerance and dependence. Factors governing release of amino acids in systems chronically exposed to opiates, however, remain undefined. Using rats, each prepared with a spinal loop dialysis catheter and with a chronic lumbar intrathecal infusion catheter connected to a subcutaneous minipump, the release of amino acids before and durin...

Recently, our laboratory demonstrated that human neuroblastoma cells (SH-SY5Y) are capable of synthesizing morphine, the major active metabolite of opium poppy. Now our experiments are further substantiated by extending the biochemical studies to the entire morphine pathway in this human cell line. L-[1,2,3-13C3]- and [ring-2',5',6'-2H3]dopa showed high isotopic enrichment and incorporation in ...

Background: Role of nitric oxide (NO) on expression of morphine conditioning using a solely classic task has been proposed previously. In this work, the involvement of NO on the expression of opioid-induced conditioning in the task paired with an injection of naloxone was investigated. Methods: Conditioning was established in adult male Wistar rats (weighing 200-250 g) using an unbiased procedu...

Background and Objective: Datura stramonium L. is a medicinal herb from the family of Solanaceae. It has been used in herbal remedies for promoting health and treating several diseases. The current study was set up to compare the effects of Datura stramonium L. extract on the naloxone-precipitated opiate-withdrawal in mice. Materials and Methods: Male BALB/c mice (30–35 g, n = 40) were arbitra...

AND BRENDA COLASANTI 23 Acetylcholinesterase Inhibition and Morphine Effects in Morphine Tolerant and Dependent Mice. HEMENDRA N. BHARGAVA AND E. LEONG WAY 31 Studies on the Transport of Morphine into the Cerebrospinal Fluid of Rabbits. J. H. WANG AND A. E. TAKEMORI 41 The Effect of 6-Hydroxydopamine on Morphine Tolerance and Physical Dependence. GLADYS FRIEDLER, HEMENDRA H. BHARGAVA, RAYMOND Q...

Repeated administration of morphine sensitizes animals to the stimulant and rewarding properties of the drug. It may also alter the expression and function of GABA receptors within the ventral tegmental area (VTA), a midbrain region that is implicated in morphine action. In the present study, the effect of activation of GABAB receptor subtypes within the VTA on morphine-induced incentive sensit...

Repeated administration of morphine sensitizes animals to the stimulant and rewarding properties of the drug. It may also alter the expression and function of GABA receptors within the ventral tegmental area (VTA), a midbrain region that is implicated in morphine action. In the present study, the effect of activation of GABAB receptor subtypes within the VTA on morphine-induced incentive sensit...

Neuropeptide FF (NPFF) modulates opiate actions. It has pro-nociceptive effects, primarily through the NPFF receptor 1 subtype, and anti-nociceptive effects, primarily through the NPFFR2 subtype. AC-263093 is a small l, organic, systemically active molecule that was previously shown to functionally activate NPFFR2, but not NPFFR1. It was hypothesized that AC-263093 would attenuate morphine tole...

AND BRENDA COLASANTI 23 Acetylcholinesterase Inhibition and Morphine Effects in Morphine Tolerant and Dependent Mice. HEMENDRA N. BHARGAVA AND E. LEONG WAY 31 Studies on the Transport of Morphine into the Cerebrospinal Fluid of Rabbits. J. H. WANG AND A. E. TAKEMORI 41 The Effect of 6-Hydroxydopamine on Morphine Tolerance and Physical Dependence. GLADYS FRIEDLER, HEMENDRA H. BHARGAVA, RAYMOND Q...

BACKGROUND The authors examined the hypothesis that epidural administration of local anesthetic, in contrast to epidural analgesia with morphine, inhibits postoperative protein oxidation during administration of glucose. METHODS Fourteen patients were randomly assigned to undergo a 6-h stable isotope infusion study (3 h fasted, 3 h feeding with 4 mg.kg(-1).min(-1) glucose) on the second day a...