Oxidative decarboxylation and transamination of 1-14C-branched chain amino and alpha-keto acids were examined in mitochondria isolated from rat heart. Transamination was inhibited by aminooxyacetate, but not by L-cycloserine. At equimolar concentrations of alpha-ketoiso[1-14C]valerate (KIV) and isoleucine, transamination was increased by disrupting the mitochondria with detergent which suggests...

It has been demonstrated (1) that the oxidation-reduction of methylglyoxal to lactic acid occurs in two steps. The first step is a condensation of methylglyoxal and reduced glutathione to a thiol ester which is hydrolytically cleaved to lactic acid and reduced glutathione. It was pointed out that such a mechanism might be operative in other aldehyde-oxidizing systems such as glyceraldehyde phos...

Palladium (II) coordination complexes catalyze the reaction of alcohols with ketones to yield ethers. During the catalytic cycle, the alcohol adds selectively to the β-carbon (anti-Markovnikov). In this work, mechanism and kinetics for the reaction of methanol with methyl vinyl ketone (MVK), being catalyzed by Pd, has been theoretically investigated in detail. Using quantum mechanical approach,...

The effect of the long chain keto acid, which is one of the secondary products from oxidative deteriolation of lipids, on the enzyme activity was investigated in vitro. Cholinesterase and succinate dehydrogenase were inhibited more effectively by 12-keto oleic acid than by linoleic acid, whereas the inhibition of trypsin by linoleic acid was greater than that caused by 12-keto oleic acid. This ...

By oxidation of P-keto acids, cyclopropanols, and acyl chromate complexes with tris(2-pyridinecarboxylato)manganese(III) (Mn(pic)g), a-keto, p-keto, and alkyl radicals are generated respectively, which react with olefinic compounds to afford intermolecular addition products in good yield. The cation radicals can be generated from a-stannylalkyl sulfides, (1 -stannylalkyl)amino compounds, and a-...

Plasma ketoconazole (KETO), amprenavir (APV), and ritonavir (RTV) pharmacokinetics were evaluated in 15 healthy subjects after being treated with KETO at 200 mg once daily (QD), fosamprenavir (FPV)/RTV at 700/100 mg twice daily (BID), and then KETO at 200 mg QD plus FPV/RTV at 700/100 mg BID in this open-label study. The KETO area under the concentration-time curve at steady state was increased...

Background: The organic matter (OM) in soils interacts with polyvalent cations such as Ca2+ through hydroxyl (OH), carboxylic acid, ester, keto, aldehyde (summarized C=O), and carboxylate (COO?) functional groups. Such interactions affect the bonding strength of double bond between C O atom groups, which is assumed to shift wavenumber (WN) region O–H (hydroxyl), C=O, COO?, OMcat (i.e., C=O inte...

Capillary Gas Chromatography (CGC) is capable of determining underivatized cyclophosphamide (CPA) using SCOT OV 275 columns. Then CPA is subjected to in situ degradation resulting in formation of a cyclization product which can be determined selectively in biological fluids. In routine bioanalysis however cyclization products of CPA metabolites might interfere, e.g. 4-keto CPA. In the present s...